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脊髓星形胶质细胞机械性损伤的体外培养模型
1.材料与方法 取新生的wistar 大鼠脊髓,剪碎,0125 %胰蛋白酶消化1h ,用含血清的DMEM培养液终止胰蛋白酶的作用; 而后吹打成散在的单个细胞,进
2025-01-14 7 -
探索RACE实验在疾病相关基因研究中的应用价值
1}克隆疾病相关基因的全长序列RACE实验能够高效地从mRNA的3'端或5'端扩增出未知的cDNA序列，从而填补基因序列的空白。在疾病相关基因研
2025-01-14 10 -
RACE实验优化策略：提升实验成功率的实用技巧
一、引物设计与选择⑴引物长度与GC含量：引物长度建议控制在23-28个核苷酸之间，不超过30个。GC含量应保持在50%-70%之间，以确保引物的稳定性和扩增
2025-01-14 10 -
mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridizatio
Two methods are commonly employed to characterize the spatiotemporal aspect of g
2025-01-14 9 -
RACE实验有哪些发展趋势？
一、技术本身的优化与创新【提高特异性和灵敏度】：随着分子生物学技术的不断发展，RACE实验将不断优化其引物设计、反转录和PCR扩增策略，以提高实验的特异性和
2025-01-14 10 -
Directing Human Embryonic Stem Cells to a Retinal Fate
Substantial progress has been made in the development of methods to direct embry
2025-01-14 10 -
Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests
Infants born prematurely have an enhanced intestinal permeability compared to he
2025-01-14 12 -
Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interac
The dynamics of proteins play a key role in the organization and control of nucl
2025-01-14 8 -
Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
Cloning using somatic cell nuclear transfer (SCNT) may be a useful tool for cons
2025-01-14 11 -
Cryo-electron Tomography of Microtubules Assembled In Vitro from Purified Components
Cryo-electron tomography of vitrified specimens allows visualization of thin bio
2025-01-14 8 -
TUNEL labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling by Boehringer Mannhei
2025-01-14 11 -
RACE实验在基因克隆中的应用：从理论到实践的跨越
一、RACE实验的理论基础RACE技术是一种基于mRNA反转录和PCR技术的分子生物学方法。其原理是利用已知的部分cDNA序列，通过设计特异性引物，结合逆转录和
2025-01-14 11 -
Quantification of Protein Carbonylation
Protein carbonylation is the most commonly used measure of oxidative modificatio
2025-01-14 6 -
Plasmid DNA Purification Via the Use of a Dual Affinity Protein
Methods are presented for the production, affinity purification and analysis of
2025-01-14 7 -
RACE实验深度探索：从原理到应用的全面解析
——RACE技术的原理 RACE技术的核心在于利用已知的部分cDNA序列，通过逆转录和PCR扩增，获得完整的cDNA的5′和3′端序列。这一过程分为3′RAC
2025-01-14 10
