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Viral Infection Protocols
This chapter describes the protocol for preparation of recombinant adenoviruses
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与传统染色相比，考马斯亮蓝染色有哪些具体优势？
【一】灵敏度高考马斯亮蓝染色法的灵敏度显著高于传统染色方法，如Lowry法等。其最低蛋白质检测量可达微克级，甚至能检出0.1μg的蛋白量，这对于微量蛋白质的检测
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Purification of Drosophila Protein Complexes for Mass Spectrometry
Drosophila melanogaster is one of the best characterized model systems for genet
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Rhythmic Conidiation in Neurospora crassa
In the filamentous fungus Neurospora crassa the production of asexual spores (co
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考马斯亮蓝染色后，如何保存凝胶？
1）置于保存液中保存染色并脱色至获得清晰的蓝色条带和干净背景后，可将凝胶放置在7%的乙酸中保存。这种方法可以有效地固定凝胶上的蛋白质，防止其降解或脱落，同时保持
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Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic
The epidermis is a complex tissue composed principally of differentiated keratin
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Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
Several types of DNA lesions are formed on irradiation of cells with ultraviolet
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如何正确进行考马斯亮蓝染色？
一、实验准备首先，你得准备好这些“主角”和“配角”：SDS-PAGE电泳分离后的蛋白质凝胶、考马斯亮蓝染色液、去染液、染色容器、水平摇床等。这些可都是染色过程中
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Transmitochondrial Cybrids: Tools for Functional Studies of Mutant Mitochondria
Mitochondrial functions are controlled by both mitochondrial DNA (mtDNA) and nuc
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Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediat
This chapter provides information about the oncoretroviral transduction of human
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细胞凋亡与细胞坏死的的区别
相关专题 细胞的作用 细胞凋亡专题 生命活动的基本单位——细胞细胞 程序性死亡 概念：细胞程序死亡(programmed Cell death，PCD)也常常被
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Separation and Maintenance of Primary T and B Lymphocytes
Two distinct populations of lymphocytes have been identified: T lymphocytes, whi
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The Extraction and Affinity Chromatography of Messenger RNA
Living cells contain two forms of nucleic acid-deoxyribonucleic acid (DNA) and r
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考马斯亮蓝染色常见问题解析
一、染色不均匀问题解析：染色不均匀可能是由于样本未固定或清洗干净，染色液浓度不均，染色温度不适宜，或染色时间不够等导致的。解决方案：①确保样本已经固定并
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Methods for Culturing Mouse and Human Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 yea
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