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Measurement of Wee Kinase Activity
The Wee kinases (Wee1, Wee2, and Myt1) are major regulators of mitotic entry. Th
2025-01-08 4 -
Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of
The white adipose tissue stores energy in the form of triglycerides in time of n
2025-01-08 6 -
Techniques for the Measurement of Nitric Oxide
Nitric oxide (NO) is an important biological mediator involved in control of blo
2025-01-08 4 -
细胞转染后怎么选择抗生素
相关专题 总有一种转染方法适合你hangcy2004问:细胞转染 后建立稳转细胞系大多用G418来杀,可是这种抗生素的选择是根据什么来确定的呢?还可以用其他的抗
2025-01-08 8 -
粗线期(pachytene stage, pachynema)
前期I的第三个阶段,又称重组期(recombination stage)。该阶段开始于同源染色体联会之后,染色体明显变粗变短(至少缩短了四分之一),结合紧密,此
2025-01-08 15 -
End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA
Polymerase chain reaction (PCR) is an important qualitative procedure in the rou
2025-01-08 10 -
Pharmacology, Biodistribution, and Efficacy of GPCR-Based Pepducins in Disease Models
G protein-coupled receptors (GPCR) are a superfamily of receptors that are vital
2025-01-08 8 -
2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics
2D- and 3D-Dynamic Image Analysis Systems (2D- and 3D-DIAS) for quantitative ana
2025-01-08 7 -
In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells
Adult bone marrow stem cell is an ideal target for gene therapy of genetic disea
2025-01-08 8 -
Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clo
Owing to the nearly limitless diversity of immunoglobulin (Ig) variable-region g
2025-01-08 8 -
Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry
Imaging mass spectrometry (IMS) provides a unique method to probe for chemical d
2025-01-08 6 -
Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
In this chapter, we describe our methods to evaluate the dynein heavy chain gene
2025-01-08 4 -
新型核酸分子荧光探针——分子信标的原理及应用
分子信标(molecular beacon)是一种具有发夹结构的新型荧光标记核酸探针,具有高灵敏度、高特异型,其在聚合酶链反应(PCR)、核酸序列的分析、活细胞
2025-01-08 22 -
做无菌实验前,你需要做好这些准备
工作区域尽可能地远离通风口和交通口。不应该开窗工作,也不要靠近门口。通风橱能够帮助无菌状态的保持,但它也不一定完全保证。确保整洁的工作环境。拿走所有不用的仪器和
2025-01-08 13 -
RNA沉淀方法
乙醇沉淀RNA通常RNA 可以像DNA 一样沉淀。步骤1. 加入1/10 体积1 mol/L 的NaOAc (pH4. 8) 和2.5 倍体积冷的95 %的乙醇
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