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  • Measurement of Wee Kinase Activity

    Measurement of Wee Kinase Activity

    The Wee kinases (Wee1, Wee2, and Myt1) are major regulators of mitotic entry. Th

    2025-01-08 4
  • Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of

    Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of

    The white adipose tissue stores energy in the form of triglycerides in time of n

    2025-01-08 6
  • Techniques for the Measurement of Nitric Oxide

    Techniques for the Measurement of Nitric Oxide

    Nitric oxide (NO) is an important biological mediator involved in control of blo

    2025-01-08 4
  • 细胞转染后怎么选择抗生素

    细胞转染后怎么选择抗生素

    相关专题 总有一种转染方法适合你hangcy2004问:细胞转染 后建立稳转细胞系大多用G418来杀,可是这种抗生素的选择是根据什么来确定的呢?还可以用其他的抗

    2025-01-08 8
  • 粗线期(pachytene stage, pachynema)

    粗线期(pachytene stage, pachynema)

    前期I的第三个阶段,又称重组期(recombination stage)。该阶段开始于同源染色体联会之后,染色体明显变粗变短(至少缩短了四分之一),结合紧密,此

    2025-01-08 15
  • End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA

    End-Point Titration-PCR for Quantitation of Cytomegalovirus DNA

    Polymerase chain reaction (PCR) is an important qualitative procedure in the rou

    2025-01-08 10
  • Pharmacology, Biodistribution, and Efficacy of GPCR-Based Pepducins in Disease Models

    Pharmacology, Biodistribution, and Efficacy of GPCR-Based Pepducins in Disease Models

    G protein-coupled receptors (GPCR) are a superfamily of receptors that are vital

    2025-01-08 8
  • 2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics

    2D and 3D Quantitative Analysis of Cell Motility and Cytoskeletal Dynamics

    2D- and 3D-Dynamic Image Analysis Systems (2D- and 3D-DIAS) for quantitative ana

    2025-01-08 7
  • In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells

    In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells

    Adult bone marrow stem cell is an ideal target for gene therapy of genetic disea

    2025-01-08 8
  • Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clo

    Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clo

    Owing to the nearly limitless diversity of immunoglobulin (Ig) variable-region g

    2025-01-08 8
  • Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry

    Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry

    Imaging mass spectrometry (IMS) provides a unique method to probe for chemical d

    2025-01-08 6
  • Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena

    Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena

    In this chapter, we describe our methods to evaluate the dynein heavy chain gene

    2025-01-08 4
  • 新型核酸分子荧光探针——分子信标的原理及应用

    新型核酸分子荧光探针——分子信标的原理及应用

    分子信标(molecular beacon)是一种具有发夹结构的新型荧光标记核酸探针,具有高灵敏度、高特异型,其在聚合酶链反应(PCR)、核酸序列的分析、活细胞

    2025-01-08 22
  • 做无菌实验前,你需要做好这些准备

    做无菌实验前,你需要做好这些准备

    工作区域尽可能地远离通风口和交通口。不应该开窗工作,也不要靠近门口。通风橱能够帮助无菌状态的保持,但它也不一定完全保证。确保整洁的工作环境。拿走所有不用的仪器和

    2025-01-08 13
  • RNA沉淀方法

    RNA沉淀方法

    乙醇沉淀RNA通常RNA 可以像DNA 一样沉淀。步骤1. 加入1/10 体积1 mol/L 的NaOAc (pH4. 8) 和2.5 倍体积冷的95 %的乙醇

    2025-01-08 21