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Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems
Fluorescence correlation spectroscopy (FCS) is an emerging technique employed in
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Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology
The recently developed near-infrared (NIR) light imaging technology combines low
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Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Ind
Human embryonic stem cells efficiently differentiate blood vessels, which allows
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Phylogenomic Analysis by Chromosome Sorting and Painting
Chromosome sorting by flow cytometry is the principle source of chromosome-speci
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Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Hel
Mitochondrial DNA (mtDNA) maintenance can be and has been studied in a wide vari
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High Cell-Density Fermentation
The purpose of this chapter is to provide sufficient instruction for the reader
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Locomotor Activity in Rodents
Many of the behavioral parameters exhibited by an organism show daily fluctuatio
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Chemical Induction of Apoptosis
Chemical Induction of Apoptosis - 1 May 2001p53, p21WAF1, Myc, Bcl-2, Bax, Bcl-x
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Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw
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Co-IP免疫共沉淀实操注意事项
免疫共沉淀是一种以抗体和抗原识别专一性为基础，用于研究蛋白质相互作用的经典方法。我们在进行实验的操作时要注意的有：1、样品制备时要注意：细胞裂解要采用温和的裂解
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Co-IP免疫共沉淀实验常见问题及解决方法
Co-IP免疫共沉淀常见问题及解决方法：一、实验结果高背景原因1：制备样品中可能有不完全溶解的大的蛋白复合体制备样品后进行短暂超声处理（3次，每次5秒钟），然后
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Northern Blot印迹杂交实验介绍
Northern印迹杂交（Northern blot）。这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法。DNA印迹技术由Southern于1975年创
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Northern Blot印迹杂交实验详细操作步骤
Northern Blot实验详细操作步骤：1、总RNA提取。2、变性胶的制备：取琼脂糖0.2g，加入DEPC H2O12.4 ml，加热融化，于保温状态下加入
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Southern Blot印迹杂交实验介绍
Southern印迹杂交是进行基因组DNA特定序列定位的通用方法。其基本原理是：具有一定同源性的两条核酸单链在一定的条件下，可按碱基互补的原则特异性地杂交形
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Southern Blot印迹杂交实验操作步骤
一、Southern Blot待测核酸样品的制备1、制备待测DNA基因组DNA是从动物组织（或）细胞制备。1.采用适当的化学试剂裂解细胞，或者用组织匀浆器研磨破
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