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Analysis of mRNA Expression During Preimplantation Development
Preimplantation-stage mammalian embryos are very simple morphologically, being c
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裸鼠皮下成瘤实验常见问题处理方法
1、这个实验为什么用裸鼠而不用普通的老鼠或者小白鼠?裸鼠成瘤实验用的裸鼠,最大的特点是没有胸腺等免疫器官,它的免疫力几乎为0,对肿瘤细胞没有免疫作用,易于成瘤。
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Histological Processing of Teeth and Periodontal Tissues for Light Microscopy Analysis
It is possible to obtain histological preparation of teeth and periodontium with
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冰冻切片实验介绍
冰冻切片(frozen section)是一种在低温条件下使组织快速冷却到一定硬度,然后进行切片的方法。其种类较多,有低温恒冷箱冰冻切片法、二氧化碳冰冻切片法、
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Infection with retroviruses
Infection with retrovirusesIt is well established with avian retroviruses that c
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冰冻切片详细操作步骤
冰冻切片操作步骤一:取材要进行冰冻切片观察的组织,应尽可能采取新鲜的材料,防止组织发生死后变化,影响后期染色分析结果。冰冻切片的步骤二:速冻1、将取好的组织块平
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General Linear Models
This chapter presents the general linear model as an extension to the two-sample
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Chromatin Immunoprecipitation Technique for Study of Transcriptional Dysregulation in Intact Mouse B
Transcriptional dysregulation has emerged as an important pathologic mechanism u
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冰冻切片操作注意事项
首先是冰冻切片实验制备的注意事项:1、新鲜组织的制备组织尽可能新鲜、骤冷愈快愈好,才能避免冰晶,常用的骤冷剂有:①CO2气(-78℃)②丙酮干冰冷却的轻石油醚、
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High-Throughput Screen for Aromatic Hydroxylation
The oxidation of aromatic compounds is important in producing chemical intermedi
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Evaluation of Circulating Endothelial Precursor Cells in Cancer Patients
Results obtained from preclinical studies have shown that endothelial progenitor
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冰冻切片制片的影响因素
1、组织取材对制片的影响组织取材大小:组织块大小要适宜,冷冻切片机内切片刀移动的范围有一定的限制,超出其范围时,易致切片不全,一般情况下,冰冻切片的组织大小为1
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冰冻切片与石蜡切片的区别
首先我们来看看什么是石蜡切片和冰冻切片:石蜡切片:石蜡切片是组织学常规制片技术中最为广泛应用的方法。石蜡切片不仅用于观察正常细胞组织的形态结构,也是病理学和法医
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酵母遗传学方法11:PCR介导基因破坏法
PCR 介导基因破坏法 1. 反应混合物:5 μ l 10 Taq 缓冲液5 μ l 25mmol/L MgC
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什么是HE染色法?
那么HE染色为什么应用如此广泛,它的原理是什么呢?HE染色原理是易于被碱性或酸性染料着色的性质称为嗜碱性和嗜酸性;而对碱性染料和酸性染料亲和力都比较弱的现象称为
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