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Microscale Thermophoresis for the Assessment of Nuclear Protein-Binding Affinities
The rapid advance in our knowledge of cellular regulatory mechanisms, including
2025-02-11 17 -
Cryopreservation of Human Red Blood Cells
The red blood cell (RBC), or erythrocyte, is a flexible biconcave disc 8 �m in d
2025-02-11 19 -
A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
A variety of techniques can be used to find protein partners, including immunopr
2025-02-11 11 -
Immobilization of Enzymes on Glyoxyl Agarose: Strategies for Enzyme Stabilization by Multipoint Atta
Immobilization of enzymes on pre-existing supports containing small aliphatic al
2025-02-11 10 -
Bovine Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a di
2025-02-11 15 -
Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells
The membrane organelles of an organized tissue, such as rat liver, that will alw
2025-02-11 14 -
Migration Assays for Oligodendrocyte Precursor Cells
In the developing central nervous system (CNS), oligodendrocyte precursor cells
2025-02-11 13 -
self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses
The investigation of cellular processes on the molecular level is important to u
2025-02-11 22 -
Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy
Fluorescence in situ hybridization (FISH) shows that DNA encoding ribosomal RNA
2025-02-11 17 -
Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: T
The studies of molecular events that occur in single cell types within a tissue
2025-02-11 15 -
Plant Mitochondrial Transcriptomics by Quantitative RT-PCR
Transcriptomic analysis using quantitative reverse transcriptase polymerase chai
2025-02-11 17 -
Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion
Mesenchymal stem cells are typically enriched from bone marrow via their prefere
2025-02-11 14 -
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Steroid hormones bind to their specific nuclear receptor protein, which are boun
2025-02-11 10 -
Specific PCR Primers for the Identification of Endomycorrhizal Fungi
The sensitivity of the polymerase chain reaction (PCR) (1 ,2 ), coupled to the s
2025-02-11 16 -
Combined Total Proteomic and Phosphoproteomic Analysis of Human Pluripotent Stem Cells
Despite advances in understanding pluripotency through traditional cell biology
2025-02-11 19
