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Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes
Understanding how human oocytes execute chromosome segregation is of paramount i
2025-02-11 13 -
Free-Hand Bisection of Mouse Oocytes and Embryos
Mouse oocytes and zygotes are semitransparent and large cells approximately 80 μ
2025-02-11 11 -
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients w
2025-02-11 11 -
Measurement of Apoptotic Cell Clearance In Vitro
The phagocytic clearance of apoptotic cells is a highly efficient and non-phlogi
2025-02-11 13 -
Adenoviral Transduction of Mesenchymal Stem Cells
Intact or genetically manipulated mesnechymal stem cells (MSCs) are being consid
2025-02-11 10 -
Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells
With the near completion of the human genome sequencing effort, it is now possib
2025-02-11 12 -
Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies: Real-Time Detection
Accurate detection of gene sequences in single cells is the ultimate challenge t
2025-02-11 10 -
Essentials of Recombinase-Based Genetic Fate Mapping in Mice
Fate maps, by defining the relationship between embryonic tissue organization an
2025-02-11 12 -
Assessing Chronological Aging in Bacteria
Bacteria, which are often considered as avid reproductive organisms under consta
2025-02-11 8 -
Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Mem
Cell adhesion between cells and with the extracellular matrix (ECM) results in d
2025-02-11 8 -
Analysis of Lipoplex Structure and Lipid Phase Changes
Efficient delivery of genetic material to cells is needed for tasks of utmost im
2025-02-11 15 -
In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections
A method is described for in situ hybridization of riboprobes to free-floating b
2025-02-11 11 -
Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
In situ hybridization (ISH) has significantly advanced the study of gene structu
2025-02-11 9 -
Induction of Deoxyribonucleic Acid Damage by Irradiation
γ Radiation is an electromagnetic radiation with wavelengths in the range of 0.1
2025-02-11 14 -
Messenger RNA Turnover in Cell-Free Extracts from Higher Eukaryotes
Two groups of observations support the notion that mRNA turnover influences gene
2025-02-11 11
