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Targeted Gene Delivery by Virosomes*
In 1990, the first federally approved clinical trials for treatment of a genetic
2025-01-06 9 -
Classical Genetic Manipulation
A significant advantage of Pichia pastoris as an experimental system is the abil
2025-01-06 3 -
Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets
Megakaryocytes (MK) are hematopoietic cells present in the bone marrow that are
2025-01-06 8 -
in situ Hybridization Protocols for Detection of Viral DNA Using Radioactive and Nonradioactive DNA
in situ hybridization can provide accurate intracellular localization of specifi
2025-01-06 7 -
Assays for the Calcium Sensitivity of Desmosomes
Epithelial cells in vivo exist as confluent cell sheets, but this confluence is
2025-01-06 7 -
PCR扩增产物出现杂带怎么办？
可能的原因和对应的解决方案如下：1.引物用量偏大，引物的特异性不高。应调换引物或降低引物的使用量。2.循环的次数过多。适当增加模板的量，减少循环次数。3.酶的用
2025-01-06 9 -
Clinical Pharmacology and Use of Microtubule-Targeting Agents in Cancer Therapy
The microtubule-targeting agents have made significant contributions to cancer t
2025-01-06 8 -
Whole mount TUNEL analysis of Xenopus embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8)
2025-01-06 7 -
Western blot实验最常见的10个问题及解决方案
1、Western Blot原理Westernblot的原理是蛋白质在电力场的作用下，由大到小的进行排列，利用电泳进行分离和富集。拥有抗原表位的蛋白质分子被抗体
2025-01-06 8 -
Class II MHC Antigen Processing in Phagosomes
Phagocytic antigen-presenting cells (APCs) are involved in innate and adaptive i
2025-01-06 5 -
分子伴侣(molecular chaperones)
分子伴侣是细胞中一大类蛋白质, 是由不相关的蛋白质组成的一个家系，它们介导其它蛋白质的正确装配，但自己不成为最后功能结构中的组分。分子伴侣的概念有三个特点:①凡
2025-01-06 5 -
慢病毒感染细胞后，细胞长满48小时是否可以先传代，再等到72小时再喷霉素筛选吗
通常在进行慢病毒感染后，建议遵循以下操作步骤：感染后观察细胞状态：感染后一般在48-72小时内可以看到较为明显的表达，但是否开始筛选需要看细胞的状态和感染效率。
2025-01-06 11 -
Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potentia
Embryonic stem (ES) cells are pluripotent cells derived from blastocyst-stage em
2025-01-06 7 -
Synchronization of Yeast
The budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces
2025-01-06 4 -
Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to
Electrophoretic mobility shift assay and Western blot are simple, efficient, and
2025-01-06 7
