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小鼠白细胞介素22(IL-22)酶联免疫分析
小鼠白细胞介素22(IL-22) 酶联免疫 分析试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相关液体样本中白细胞介素22(IL-
2025-01-06 8 -
Western blot 中Tween-20的作用是什么?应该怎么用?
Western blot的洗液TBST是实验室常用的配制液体,对于TBST的配方也基本上相残不多,其中配方中就有一种组分是Tween-20,一种黄色或琥珀色澄明
2025-01-06 20 -
Cell Lineage Analysis in Xenopus Embryos
Cell lineage studies reveal what kinds of tissues descend from a single cell or
2025-01-06 6 -
Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration
The function of many glycoproteins is directly related to the structure of their
2025-01-06 8 -
蛋白质的保存方法如何选择?
保存方法的选择取决于几个因素,包括蛋白质内在的稳定性、所需的保存时间的长短和对于防止破坏所要求的程度。这些因素有很大的变异性,但是,在大多数情况下都应当遵守以下
2025-01-06 9 -
胞质膜(cytoplasmic membrane)
存在于细胞质中各膜结合细胞器中的膜,包括核膜、内质网膜、高尔基体膜、溶酶体膜、线粒体膜、叶绿体膜、过氧化物酶体膜等。
2025-01-06 6 -
ChIP超详细实验步骤
ChIP的一般流程:甲醛处理细胞—收集细胞,超声破碎—加入目的蛋白的抗体,与靶蛋白-DNA复合物相互结合—加入ProteinA,结合抗体-靶蛋白-DNA复合物,
2025-01-06 10 -
Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase
Cyclic nucleotide phosphodiesterases (PDE s) include a large group of structural
2025-01-06 9 -
Sequence Analysis of the Whole Mitochondrial Genome and Nuclear Genes Causing Mitochondrial Disorder
The diagnosis of mitochondrial disorders has increased considerably over the pas
2025-01-06 9 -
粘着连接(adherens junctions, zonula adherens)
在斑块连接中,如果连接作用涉及细胞质中的骨架部分是肌动蛋白,这种连接方式称为粘着连接。根据粘着连接涉及的是两个细胞间的连接还是细胞与细胞外基质的连接,又分为两种
2025-01-06 11 -
Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle
The nuclear envelope is a complex membrane-protein system that is notoriously di
2025-01-06 6 -
Quantitative Determination of Collagen Crosslinks
The primary functional role of collagen is as a supporting tissue and it is now
2025-01-06 7 -
Telomerase Inhibition and Telomere Targeting in Hematopoietic Cancer Cell Lines with Small Non-Nucle
Telomere maintenance has been shown to be essential for unlimited growth potenti
2025-01-06 8 -
ELISA不得不懂的实验原理
ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性,酶标记的抗原或抗体既保留其免疫学活性,又保留酶的活性
2025-01-06 8 -
mRNA的分离纯化原理
【实验原理】真核生物的mRNA分子是单顺反子,是编码蛋白质的基因转录产物。真核生物的所有蛋白质归根到底都是mRNA的翻译产物,因此,高质量mRNA的分离纯化是克
2025-01-06 8