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Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
The Ras/mitogen-activated protein kinase (MAPK) pathway is essential in regulati
2025-02-11 8 -
粘着带(adhesion belt)
粘着带连接位于上皮细胞紧密连接的下方, 靠钙粘着蛋白同肌动蛋白相互作用, 将两个细胞连接起来。粘着带处相邻细胞质膜的间隙为20～30nm, 介于紧密连接和桥粒之
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Entrapment in Calcium Alginate
Entrapment of enzymes and/or cells in alginate is one of the simplest methods of
2025-02-11 14 -
大鼠成纤维细胞生长因子（FGF）酶联免疫分析
大鼠成纤维细胞生长因子（FGF ） 酶联免疫分析试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中成纤维细
2025-02-11 8 -
Genetic Complementation Protocols
Genetic selection provides a powerful tool for the study of cellular processes.
2025-02-11 10 -
An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics
The use of an in vitro system based on primary cultures of Sertoli cells isolate
2025-02-11 9 -
Radioligand-Binding Methods for Membrane Preparations and Intact Cells
The radioligand-binding assay is a relatively simple but powerful tool for study
2025-02-11 10 -
Quantifying Amplicons with ELISA
Among the numerous assays proposed for quantifying specific nucleic-acid sequenc
2025-02-11 15 -
Isolation of Microtubules (Bovine Brain)
LEVEL IIMaterials Freshly removed bovine brain 2Wire sieve (tea strainer)Microtu
2025-02-11 11 -
Screening for Thermostability
Enzyme thermostability is a property of great importance in the era of designed
2025-02-11 11 -
外周血培养基的配制
相关专题 一、配制用水 培养基的大部分是水，所以水的质量直接影响培养基的质量。按Waymouth标准，水的电阻应为200万欧姆，一般实验室里以玻璃蒸馏器制备
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cck8法检测细胞增殖详细操作步骤
以cck8法检测细胞的增殖能力为例：一、准备cck8检测细胞增殖实验器材实验仪器：CO2恒温培养箱、光学倒置显微镜、超净工作台、血球计数板实验试剂与耗材：DME
2025-02-10 31 -
细胞增殖检测之MTT法
首先是MTT法检测细胞增殖的原理：MTT为黄色化合物，是一种接受氢离子的染料，可作用于活细胞线粒体中的呼吸链，在琥珀酸脱氢酶和细胞色素C的作用下tetrazol
2025-02-10 28 -
EDU法检测细胞增殖操作
EdU可以说是一款革命性的细胞增殖状态检测方法与传统的免疫荧光染色(BrdU)检测方法相比，EdU只有BrdU抗体大小的1/500，在细胞内更容易扩散，不需要严
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细胞增殖检测的注意事项
一、MTT法检测细胞增殖注意事项：1、MTT的配制：MTT对菌和光十分敏感，配制过程中注意无菌环境及避光，使用超净台配制时，可以将超净台的灯光先关掉。MTT具有
2025-02-10 17
