-
Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phos
We describe a method to visualize the migration of osteoclast precursors within
2025-02-08 21 -
Methods for the Assessment of Telomere Status
Most methods for examining telomere functionality have relied on measurements of
2025-02-08 20 -
Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4
Embryonic stem (ES) cells proliferate infinitely while maintaining pluripotency.
2025-02-08 24 -
Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
Nucleotide excision repair (NER) is one of the most important systems in eukaryo
2025-02-08 17 -
Culturing Human Schwann Cells
The method involved in the establishment of human adult Schwann cell cultures ha
2025-02-08 23 -
Chemokine Receptor Dimerization and Chemotaxis
A broad array of biological responses ranging from cell polarization, movement,
2025-02-08 21 -
Application of FISH to Archival Cytogenetic Slides
Archival cytogenetic slides of unique clinical and tumorgenetic cases embedded i
2025-02-08 24 -
Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is i
One of the most important principles of scientific endeavour is that the results
2025-02-08 17 -
什么是酵母单杂实验？
酵母单杂交技术最早是1993年由Li等从酵母双杂交技术发展而来，通过对报告基因的表型检测，分析DNA与蛋白之间的相互作用，以研究真核细胞内的基因表达调控。由于酵
2025-02-08 23 -
酵母单杂交实验详细步骤
一、构建诱饵菌株1、制备YM4271感受态细胞2、取上述感受态菌液，与1μg pHisi-cor promoter质粒混匀后加入预冷为0.2cm（electro
2025-02-08 29 -
酵母杂交实验的常见问题—转化篇
问题一：菌种是否需要两次平板活化？答：根据菌种生长的具体情况。初始菌为1个月内活化过的酵母平板菌落，只需要进行一次平板活化；新鲜转化产物用于感受态制备，不需要平
2025-02-08 24 -
酵母杂交实验的常见问题—试剂篇
问题一：经典转化试剂盒和Super转化试剂盒是否需要准备其它试剂？答：需要准备DMSO，无菌水或者无菌生理盐水，无需其它试剂。问题二：50%PEG3350溶液是
2025-02-08 23 -
什么是RNA pulldown实验？
RNA pull down是用于检测RNA结合蛋白与其靶RNA之间互作的实验手段。RNA pull down使用体外转录法标记生物素RNA探针，然后与胞浆蛋白提
2025-02-08 20 -
RNA pull-down详细实验步骤
1、磁珠准备（1）取3µg Biotin标记的RNA，加入适量Structure Buffer，使得RNA形成二级结构。然后将RNA 95℃加热2 min，冰浴
2025-02-08 25 -
RNA pull-down注意事项与常见问题
一、RNA pull-down实验注意事项1、实验前期准备（1）首先是样本的收集和处理，如果样本是细胞，要考虑细胞中该RNA的表达量，如果RNA含量较低，那提取
2025-02-08 24
