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胸腹水染色体标本制备
一、原理 某些肿瘤细胞可脱落入胸腹水中，并继续生长、增值。因此，由胸腹水细胞可制备较好的染色体标本。胸腹水细胞染色体分析对恶性肿瘤具有辅助诊断的价值。如
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Approaches to Cloning of Pain-Related Ion Channel Genes
Molecular pain research is a relatively new and rapidly expanding field that rep
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ES Cell-Mediated Conditional Transgenesis
The use of mouse embryonic stem (ES) cells to alter the mouse genome has become
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细胞培养的微生物污染排除
一、抗生素除菌法：用BM－1（截耳素衍生物）和BM－2（四环素衍生物）抑制支原体1．抗生素制备：均可用PBS配成250浓缩液－20℃备用，使用浓度参考《组织培养
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Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
Lipid bodies, also known as lipid droplets, are present in most eukaryotic cells
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小白鼠染色体的制备和观察
相关专题 一、实验目的 通过实验，掌握空气干燥法制片技术。 二、实验原理 在骨髓细胞中，有丝分裂的指数是相当高的，因此可以直接得到中期细胞而不必像血淋巴细胞
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交换
在减数分裂Ⅰ的粗线期发生的同源染色体的非姊妹染色单体间的局部交换，以及连锁的基因重组。 1964年 R． Holliday从分子水平对交换做出解释，认为二价体染
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Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
Primary murine splenic B cells can be cultured ex vivo and, following treatment
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RNA Extraction From Drosophila Heads
In Drosophila , input, pacemaker, and output genes are expressed circadianly. mR
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Linearization and Purification of the Shuttle Plasmid
相关专题 1.0 Purpose 1.1 This protocol may be used for the linearization and purifi
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Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells
Semi-intact cells are cells with plasma membranes that have been permeabilized b
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内膜系统（endomembrane system）
具有界膜的细胞器如内质网、高尔基器、溶酶体、过氧化物酶体、线粒体、叶绿体等，与细胞核的核膜一起统称为内膜系统。为真核细胞所特有，是完成真核细胞生命活动过程的必需
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Detection of Tight Junction Barrier Function In Vivo by Biotin
Tight junctions (TJs) are the most apical component of the junctional complexes
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Single Cell Analysis of Lipid Rafts
Lipid rafts are plasma membrane microdomains that serve as platforms for the ass
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Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
Advances in our understanding of biology at the molecular level are very much dr
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