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SpermEgg Fusion Assay in Mammals
As representatives of the 60 trillion cells that make a human body, a sperm and
2025-02-08 12 -
Preparation, Characterization, and Use of Antioxidant-Liposomes
Antioxidant liposomes provide a unique means of delivering both water and/or lip
2025-02-08 14 -
Genetic Modification of Chondrocytes Using Viral Vectors
The use of isolated cells to construct engineered tissues provides the opportuni
2025-02-08 10 -
三联管(triplet)
见于中心粒(centrioles)和基体(basal bodies),由A、B、C三个单管组成,A管由13根原纤维组成,B管和C管都是10根原纤维,所以一个三联
2025-02-08 9 -
Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
Stem cell-mediated tissue repair is a promising approach in regenerative medicin
2025-02-08 10 -
Principles for Derivation of Human Embryonic Stem Cells
This chapter describes the principles for derivation and maintenance of human em
2025-02-08 12 -
Protocols for Studying Antizyme Expression and Function
Antizyme (AZ) is a key molecule in feedback regulation of cellular polyamines. I
2025-02-08 10 -
The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, and chondrocytes from
Localization of messenger RNA (mRNA) is a process used by eukaryotes to control
2025-02-08 12 -
Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells
The efficient generation of mesenchymal cells such as adipocytes, osteoblasts, a
2025-02-08 11 -
Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium
The intestinal barrier is formed by a monolayer of columnar epithelial cells. Th
2025-02-08 16 -
In Vitro Human T Cell Development Directed by NotchLigand Interactions
Traditionally, the study of human T cell development has relied on the availabil
2025-02-08 13 -
干细胞(stem cells, SC)
一类具有自我复制能力(self-renewing)的多潜能细胞,在一定条件下,它可以分化成多种功能细胞。干细胞有两种分类方法,一是根据干细胞所处的发育阶段分为胚
2025-02-08 16 -
Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos
The anthozoan cnidarian Nematostella vectensis has emerged as a key model system
2025-02-08 13 -
巨噬细胞培养方法与步骤
一、巨噬细胞培养设施设备与器皿1、巨噬细胞培养设施设备(1)超净工作台:也称净化工作台,分为侧流式、直流式和外流式三大类。(2)无菌操作间:一般由衣间,缓冲间和
2025-02-07 13 -
巨噬细胞分离与纯化步骤
一、细胞分离1、取6周左右的小鼠(或600克左右的豚鼠,或3公斤左右的家兔),剃去腹部的毛并消毒。腹腔注射1ml(豚鼠20ml,家兔200ml)无菌的液体石蜡或
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