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Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers
Integrins are heterodimeric cell-surface receptors involved in a variety of func
2025-02-07 9 -
Analysis of the Cell Cycle in Mouse Embryonic Stem Cells
The molecular mechanisms underlying self-renewal of pluripotent embryonic stem (
2025-02-07 12 -
Basic Bayesian Methods
In this chapter, we introduce the basics of Bayesian data analysis. The key ingr
2025-02-07 9 -
The Macrophage
Macrophages are a diverse phenotype of professional phagocytic cells derived fro
2025-02-07 14 -
In Vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche
Hematopoietic stem cells (HSC) undergo multilineage differentiation or self-rene
2025-02-07 8 -
Basic Cell Culture
This article will describe the basic techniques required for successful cell cul
2025-02-07 10 -
Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cell
This chapter describes the use of microscope-based fluorescence resonance energy
2025-02-07 7 -
Solubilization of Proteins in 2DE: An Outline
Protein solubilization for two-dimensional electrophoresis (2DE) has to break mo
2025-02-07 11 -
Recording of Ion Channel Activity in Planar Lipid Bilayer Experiments
Planar lipid bilayer is an electrophysiological technique that enables study of
2025-02-07 12 -
The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify On
Onchocerca volvulus is a filarial parasite that is the causative agent of onchoc
2025-02-07 13 -
Cripto Signaling in Differentiating Embryonic Stem Cells
Embryonic stem (ES) cells have been suggested as candidate therapeutic tools for
2025-02-07 17 -
Site-Directed Mutagenesis Using Overlap Extension PCR
Site-directed mutagenesis and the polymerase chain reaction (PCR) represent two
2025-02-07 23 -
Identification and Isolation of Rodent Respiratory Tract Dendritic Cells
This chapter describes the preparation of respiratory tract tissue from both mic
2025-02-07 11 -
Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysi
Since its introduction more than a decade ago, denaturing HPLC has been widely u
2025-02-07 16 -
The Xenopus Embryo as a Model System for Studies of Cell Migration
In this chapter, we describe procedures for the microsurgical removal of cells a
2025-02-07 19
