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Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)
We have developed protocols whereby a total of 30–90 � 106 hMSCs with an average
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In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique
Skeletal muscle mass is determined by the balance between rates of protein synth
2025-02-07 14 -
Paraformaldehyde Fixation of Cells
Paraformaldehyde Fixation of CellsBackgroundThis fixation method is good for cel
2025-02-07 10 -
Cuticle Preparation of Drosophila Embryos and Larvae
The Drosophila embryonic ventral epidermis has served as a unique tissue for the
2025-02-07 10 -
NF-kappaB信号通路(10张)
相关专题NF-kappaB signaling pathway how the ubiquitination system regulates the acti
2025-02-07 14 -
Clinical Uses of Liver Stem Cells
Liver transplantation offers a definitive cure for many liver and metabolic dise
2025-02-07 14 -
Proteomic Tools for the Analysis of Cytoskeleton Proteins
Proteomic tools have become an essential part of the tool kit of the molecular b
2025-02-07 10 -
A Tutorial on Mathematical Modeling of Biological Signaling Pathways
Mathematical models have been widely used in the studies of biological signaling
2025-02-07 11 -
带蛋白(band protein)
由两个亚基组成的球形蛋白,它在膜骨架中的作用是通过同血影蛋白结合,促使血影蛋白同肌动蛋白结合。带4.1蛋白本身不同肌动蛋白相连,因为它没有与肌动蛋白连接的位点。
2025-02-07 11 -
Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE
Defining membrane proteomes is fundamental to our understanding of many physiolo
2025-02-07 11 -
Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies
Histone modifications are central to epigenetic regulation and must be reestabli
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Identification of Disease Markers by Differential Display: Prion Disease
In order to identify molecular markers of prion disease in peripheral tissues, w
2025-02-07 12 -
LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and Meconium
We describe a single method for the detection and quantitation of 13 commonly pr
2025-02-07 13 -
肌球蛋白(myosin)
肌球蛋白是一种分子发动机, 以肌动蛋白丝作为运行的轨道。实际上, 肌球蛋白也是ATPase, 通过ATP的水解导致构型的变化从而在肌动蛋白丝上移动。这种ATPa
2025-02-07 8 -
Live-Cell Imaging of the Cytoskeleton and MitochondrialCytoskeletal Interactions in Budding Yeast
This chapter describes labeling methods and optical approaches for live-cell ima
2025-02-07 9