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Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
This chapter describes the analysis of long- and short-lived plasma cells on tis
2025-01-03 6 -
In Situ Hybridization for Metalloproteinases and Their Inhibitors
The imminent completion of the human and mouse genome projects will reveal all m
2025-01-03 11 -
Profiling the Metabolic Signature of Senescence
Aging is a complex process, which involves changes in different cellular functio
2025-01-03 10 -
COPⅡ被膜小泡(COPⅡ coated vesicle)
由外被蛋白Ⅱ(coat protein Ⅱ, COP Ⅱ )包裹的小泡。外被蛋白是一个大的复合体，称为外被体(coatomer)。这种类型的小泡介导非选择性运输
2025-01-03 6 -
Isolation and Purification of Vertebrate DNAs
Isolation of DNA from surrounding tissues is a crucial initial step in any molec
2025-01-03 10 -
Magnetic Resonance Imaging Analysis of Embryos
Analyzing phenotypic effects in embryos produced with directed gene manipulation
2025-01-03 9 -
原代培养(primary culture)
原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此，较为严格地说是指成功传代之前的培养，此时的细胞保持原有细胞的基本性质，如果是正常细胞，仍然保留二
2025-01-03 8 -
Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
This chapter covers gene expression analysis by microarray to study and characte
2025-01-03 7 -
Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Successful retroviral gene transfer into hematopoietic cells has been demonstrat
2025-01-03 7 -
In Vitro Fabrication of Engineered Human Skin
In vitro fabrication of human epidermal tissues that mimic the biochemical and m
2025-01-03 18 -
Using Somatic-Cell Nuclear Transfer to Study Aging
In mammals, a diploid genome following fertilization of haploid cells, an egg, a
2025-01-03 8 -
Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal
Regulated secretion is a central issue for the specific function of many cells;
2025-01-03 8 -
Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine
Serum and urine protein electrophoresis are used primarily to screen for the pre
2025-01-03 3 -
Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for
Injuries to the peripheral nervous system affect 1 in 1,000 individuals each yea
2025-01-03 4 -
Solid Tumor DNA Ploidy Analysis
Flow cytometric DNA content analysis of human neoplasia provides quantitative in
2025-01-03 9
