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Parasexual Genetics Using Axenic Cells
Normally, vegetative Dictyostelium grow as haploid cells. Occasionally, two hapl
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Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein
Methods are presented for the preparation, ligand density analysis and use of an
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Methods for Detecting Cells in S Phase
S phase is that period of time in the cell-division cycle during which nuclear c
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Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry
The testis is a heterogeneous organ that comprises a number of cell types, inclu
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Protein Expression Profiling
Protein expression profiling is defined in general as identifying the proteins e
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Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcripti
The mouse mammary tumor virus (MMTV) promoter has been used extensively as a mod
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A Genome-wide RNAi Screen for Polypeptides that Alter rpS6 Phosphorylation
Mammalian target of rapamycin (mTOR) is a giant protein kinase that controls cel
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b-Galactosidase Color Assay for Cultured Cells
b-Galactosidase Color Assay for Cultured Cells 1. For transient expression assay
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Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors
The central nervous system (CNS) is composed of multiple cell types formed throu
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Derivation and Maintenance of Human Embryonic Stem Cells
Since their derivation in 1998, human embryonic stem cells (hESCs) have been the
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Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES
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Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Limited understanding of the cell biology of the breast and breast cancer hamper
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Peptide Structure Determination by NMR
The difference between peptides and proteins (the subject of Chapter 2 ) is that
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A first step in defining the function of a novel gene is to determine its interactions with other ge
The difference between peptides and proteins (the subject of Chapter 2 ) is that
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Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
A first step in defining the function of a novel gene is to determine its intera
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