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Chaperone-Mediated Autophagy
Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian ce
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Direct DNA Sequencing of PCR Products Using Magnetic Beads
The use of magnetic particles in many fields of biochemistry, molecular biology,
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Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells
The balance between the quiescent hematopoietic stem cell (HSC) and the highly p
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In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases
Growth factor receptors, such as the epidermal growth factor receptor (EGFR), st
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Colorimetric Detection of Senescence-Associated Galactosidase
Most normal human cells have a finite replicative capacity and eventually underg
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纤维素(cellulose)
纤维素是由葡萄糖构成的同质多聚体，在细胞壁中，由50-60个纤维素分子形成一束，并且相互平行排列，形成长的、坚硬的微纤维，这种微纤维的直径一般约为5-15nm,
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Dynamic and Exchange Processes in Macromolecules Studied by NMR Spectroscopy
It is normal for a biological system to be in a dynamic state. The function of m
2024-12-27 8 -
Biochemical Fractionation of Drosophila Cells
This chapter describes how to perform basic biochemical fractionations of Drosop
2024-12-27 7 -
Decontamination of cells from the yeast
I Destroy yeast1. Aspirate medium and wash cell in PBS.2. Incubate c
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Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Oste
Mesenchymal stem cells (MSCs) are multipotent cells that have the potential to d
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RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis
Gene silencing approaches afford investigators the ability to gain important ins
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Analysis of Reconstituted Chromatin Using a Solid-Phase Approach
Complex experimental strategies involving in vitro reconstituted chromatin or si
2024-12-27 8 -
Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates
Extracellular signals play a central role in coordinating the growth and differe
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The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation
The reversible phosphorylation of proteins is a key mechanism whereby signalling
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Testing Membrane Interactions of CPPs
The chapter deals with some biophysical methods used for investigating CPP-induc
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