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Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable a
The vector-mediated introduction of cDNA into mammalian cells by calcium phospha
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ProteinProtein Interaction Techniques: Dissect PCP Signaling in Xenopus
The planar cell polarity (PCP) pathway is a β-catenin-independent branch of the
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Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Imm
Sea urchin eggs have been used for over a century to study fertilization, cell d
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Gel-Based and Gel-Free Proteomic Technologies
Proteomics refers to the analysis of expression, localization, functions, posttr
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Nuclear Transfer in Nonhuman Primates
The nonhuman primate is a highly relevant model for the study of human diseases,
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Nanosizing by Spatially Modulated Illumination (SMI) Microscopy and Applications to the Nucleus
In this chapter we present the method of spatially modulated illumination (SMI)
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Imaging Plasma Membrane Proteins by Confocal Microscopy
Confocal microscopy has become a widely used method in the study of plasma membr
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Homologous Gene Targeting to Study ECM Assembly
Genetically engineered mutant strains of mice have become invaluable too to deci
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Assay for Protein Modification by Poly-ADP-Ribose In Vitro
The enzymatic function of poly(adenosine diphosphate (ADP)-ribose) polymerase (P
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Quantitative PCR with Internal Standardization and OLA-ELISA Product Analysis for the p53 Tumor Supp
Over the last nine years, several quantitative polymerase chain reaction (QPCR)
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Expression of EGF and HIV Envelope Glycoprotein
This chapter reviews the expression of two proteins that are naturally secreted:
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Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry
Progression of cells through successive phases and checkpoints of the cell cycle
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Extrachromosomal Assay for DNA Double-Strand Break Repair
DNA double-strand break (DSB) repair in mammalian cells has been demonstrated to
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Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Indiv
In order to restore or to introduce a gene function integrating viral vector sys
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Direct Sequencing of PCR Products Using Chemiluminescent Detection
The following protocol describes a method that can be used for the direct sequen
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