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Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling
Tight junctions restrict the paracellular movement of ions, solutes, drugs, and
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Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries
The so-called hematopoietic stem cells from mouse and human bone marrow can be i
2024-12-25 9 -
Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) comprise a diver
2024-12-25 12 -
Quantification of Xenoprotein Electroinsertion in Mammalian Cells
Recent studies reported the so-called “electroinsertion” of proteins having a me
2024-12-25 12 -
Wound healing assay
The wound healing assay allows the researcher to study cell migration and cell i
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Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
Purification of membrane proteins of necessity requires the use of detergents to
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Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers
In tissue engineering, a variety of methods are commonly used to evaluate surviv
2024-12-25 11 -
Tracking Gene-Modified T Cells In Vivo
Identification, monitoring, and analysis of genetically modified cells in the pe
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消化系统组织学分析实验-免疫组化(荧光)确定上皮增生及细胞浸润
简介对于癌症病变的组织学分析,在早期可以通过5-溴脱氧尿嘧啶核苷(BrdU)掺入测定目的细胞的增殖能力,如胃上皮增殖及肠道干细胞向上增殖能力,推测其转化的可能性
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消化系统组织学分析实验-切片观察实验
简介在解剖学观察的基础上,可以通过对肉眼可见的病变部位进行切片染色来判断具体的病理缺陷,如癌症的浸润程度,炎症的发展程度等,也可以通过病理学评判标准对其程度进行
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小鼠采血实验-腹主动脉采血法
简介小鼠处死前大量采血可用腹主动脉采血法。使用腹主动脉采血法一次可采血达 1 mL,血样污染少,缺点是操作要求较高,需专业人员统一操作。材料与仪器器材:① 解剖
2024-12-25 16 -
小鼠采血实验-颌下静脉采血法
简介颌下静脉采血法与眼后静脉采血比较,此方法对小鼠伤害较小,反复操作的可能性大,缺点是由于血样接触到毛发,可能产生溶血。使用不同规格的针头可控制血液的量,通常可
2024-12-25 14 -
小鼠采血实验-眼后静脉采血法
简介眼后静脉采血法是当需中等量的血液而又需避免动物死亡时采用的方法。此方法小鼠可灵活采血 50~500 μl,操作稳定性强,止血容易,污染较少,因此是最普遍的一
2024-12-25 16 -
小鼠采血实验-尾静脉采血法
简介小鼠尾部有左、右两根尾静脉,如需极小量采血,如用于血糖检测时,可采用尾静脉采血法。此方法取血量很小,伤口容易愈合,连续多日操作对小鼠也没有影响。原理尾静脉采
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小鼠体温测量实验-直肠肛温测量小鼠体温
简介小鼠的体温和生理节律有很大的相关性,传统的较为简便的测量小鼠体温的方法是直肠肛温测量。因为此方法检测手段简单、设备易于准备而应用较广。但直肠肛温测量对检测人
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