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Screens for Mitochondrial Mutants in Yeast
We describe methods that allow isolation, identification, and counting of mitoch
2025-01-22 11 -
A number of different viruses interact with the cell cycle in order to subvert host-cell function an
Nonhuman primate embryonic stem (ES) cells are very important for preclinical re
2025-01-22 9 -
The Cell Cycle and Virus Infection
A number of different viruses interact with the cell cycle in order to subvert h
2025-01-22 10 -
The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference
Embryonic mouse kidneys develop well in organ culture. This, coupled with the fa
2025-01-22 15 -
斑块连接 (plaque-bearing junction)
通过细胞质膜内侧的斑块(plaque) 将细胞与细胞、细胞与细胞外基质同细胞骨架连接起来的细胞连接方式称为斑块连接(plaque-bearing junctio
2025-01-22 19 -
组织培养细胞染色体制备方法
组织细胞用于遗传学分析最常见的是体外培养的细胞株，多为恶性肿瘤细胞株，且呈贴壁生长，仅小数细胞株为悬浮生长。培养细胞有来源易得、细胞分裂率高和染色体标本制出清晰
2025-01-22 19 -
In vitro culture of embryonic lungs
Isolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fet
2025-01-22 17 -
In Vitro Generation of Lymphocytes From Embryonic Stem Cells
Lymphocytes arise during ontogeny via a series of increasingly restricted interm
2025-01-22 15 -
冰冻断裂复型(freeze-fracture replication)技术
先将生物样品在液氮中(-196℃)进行快速冷冻，防止形成冰晶。然后将冷冻的样品迅速转移到冷冻装置中，并迅速抽成真空。在真空条件下，用冰刀横切冰冻样品，使样品内层
2025-01-22 9 -
Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles
The use of enzymes in industrial or medical applications has been limited (excep
2025-01-22 16 -
Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors
T cells typically differentiate via a series of coordinated steps within the hig
2025-01-22 17 -
Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abun
Numerous methods have been published for the detection of protein using avidin–b
2025-01-22 13 -
The Application of Nuclear Magnetic Resonance to Structural Studies of Polysaccharides
Polysaccharides are ubiquitous components of living tissues. They are storage co
2025-01-22 11 -
裸鼠皮下成瘤实验常见操作问题以及解决办法（下）
一、实验动物选择问题 在裸鼠皮下成瘤实验中，选择合适的实验动物是关键。常见的问题包括：不同品系、年龄、性别的裸鼠对实验结果的影响。解决方案：确保使用健康、无特定
2025-01-22 12 -
如何巧妙设计引物？
一、了解引物的定义引物又称为寡核苷酸引物，也就是RNA，是由人工合成的，PCR引物通常是一对，引物1和引物2。由于DNA聚合酶只能从核苷酸链的5'端到3
2025-01-22 20
