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雌性小鼠生殖生物学分析实验-血清激素检测实验（雌性小鼠生殖生物学分析实验）
简介血清激素检测实验的目的是检测在促性腺激素诱导后，雌性小鼠体内相关性激素的水平是否有相应的变化。材料与仪器器材：① 针头② 注射器③ 4 ℃ 离心机④ 相关手
2024-12-24 24 -
雌性小鼠生殖生物学分析实验-生育力检测实验
简介生育力检测实验是检测雌性小鼠是否发生交配行为，检测交配后是否怀孕以及产生正常数量的后代。材料与仪器器材：① SPF 级别实验动物饲养环境② 8～10 周龄正
2024-12-24 68 -
鼻咽癌动物模型建立-二乙基亚硝胺 (DEN) 法诱发鼻咽癌
简介二乙基亚硝胺 (DEN) 法诱发鼻咽癌，指的是多次用注射器从前鼻孔灌注二乙基亚硝胺 (DEN)，从而诱发鼻咽癌。材料与仪器动物模型：体重 120 g 左右的
2024-12-24 16 -
鼻咽癌动物模型建立-二甲基胆蒽 (MC) 法诱发鼻咽癌
简介二甲基胆蒽 (MC) 法是将致癌物二甲基胆蒽直接注入鼻尖，从而诱发鼻咽癌。材料与仪器动物模型：体重 120 g 左右的大鼠，雌雄均可器材：2-3 mm 直径
2024-12-24 21 -
肺癌动物模型建立-肺癌动物模型
原理组织块移植组将直径2 mm的人肺癌新鲜标本癌组织块移植于BALB/C裸鼠背部皮下组织内，每例人肺癌新鲜标本均移植入5只裸鼠体内，成瘤者为第1代。取第1代移植
2024-12-24 19 -
肺癌动物模型建立-气管内注入苯并花、硫酸铵气溶胶、甲基胆蒽等物质法构建肺癌动物模型
简介通过向气管内注入苯并花、硫酸铵气溶胶、甲基胆蒽等物质，使动物诱发肺癌。材料与仪器器材：注射器试剂：①苯并花；②硫酸铵气溶胶；③甲基胆蒽；④乙醚。步骤构建肺癌
2024-12-24 17 -
肺癌动物模型建立-焦油沥青 (GTP) 法构建肺癌动物模型
简介焦油沥青 (GTP) 法是通过向气管内注入焦油沥青，从而诱发肺癌。材料与仪器动物模型：体重 150- 200 g 大鼠（初断乳，饲养 4 周）器材：窥耳镜、
2024-12-24 17 -
Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
The biogenesis of mitochondrial membrane proteins is an intricate process that r
2024-12-24 12 -
Methods for Assessing Autophagy and Autophagic Cell Death
Autophagic (or type 2) cell death is characterized by the massive accumulation o
2024-12-24 18 -
Cultivation of Normal Human Epidermal Melanocytes
An important approach in studies of normal, diseased, and malignant cells is the
2024-12-24 23 -
Isolation and Characterization of Human Embryonic Stem Cells
The derivation of human embryonic stem (hES) cells is a challenging procedure. T
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The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins
The availability of synchrotron radiation has transformed the status of X-ray ab
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Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
This protocol describes an in vivo assay for random and directed hemocyte migrat
2024-12-24 11 -
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
T his procedure allows detection of specific cell adhesion to glycolipids resolv
2024-12-24 12 -
Use of Multiphoton Imaging for Studying Cell Migration in the Mouse
We describe a method for studying cell motility in the living mouse using multip
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