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Detection of NF-B Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
An electrophoretic mobility shift assay (EMSA) is a common and invaluable techni
2025-01-21 10 -
Methods for Iodination of Active TGF, TGF Receptor Crosslinking and Immunoprecipitation of TGF-Recep
Radioiodinated transforming growth factors ( TGF-βs) are critical reagents for f
2025-01-21 8 -
Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging
The formation of new synapses within neuronal circuits is considered a primary m
2025-01-21 13 -
Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endot
Human embryonic stem cells (hESCs) represent a promising source of cells for mod
2025-01-21 16 -
Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation
DNA methylation and histone modifications are key regulatory mechanisms in cellu
2025-01-21 13 -
Methods for Analysis of Ligand-Induced RNA Conformational Changes
Encoded within many RNA sequences is the requisite information for folding of in
2025-01-21 15 -
Sources and Derivation of Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner
2025-01-21 14 -
Master Cell Bank
Master Cell BankAuthor: Nanci DonackiSource: Contributed by Nanci DonackiAbstrac
2025-01-21 11 -
PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent D
We describe in situ hybridization protocols using peptide nucleic acid (PNA) as
2025-01-21 9 -
Recording Ion Channels in Isolated, Split-Opened Tubules
Ion channels play key roles in physiology. They function as protein transducers
2025-01-21 8 -
Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test
The number of techniques to identify, quantify and characterise cell death is ra
2025-01-21 9 -
Tiny Molecular Beacons for in vivo mRNA Detection
The molecular beacon technology is an established approach for visualizing nativ
2025-01-21 15 -
Saponin Lysis of RBCs
CuratorsJingyang Chen, Seattle Biomedical Research Institute, 307 Westlake Ave N
2025-01-21 8 -
Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders
One of the possible applications of human induced pluripotent stem (iPS) cells i
2025-01-21 9 -
Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO
The gross majority of classical apoptotic hallmarks can be rapidly examined by m
2025-01-21 9
