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体细胞核移植技术制备转基因动物-利用体细胞核移植技术制备转基因猪
材料与仪器器材：①注射器②无菌手术器械③细胞培养皿④移卵针⑤37 ℃、5％CO2 培养箱⑥倒置显微镜、体视镜⑦PCR 仪、电转仪、离心机试剂：①材料：猪②孕马血
2024-12-21 17 -
胚胎移植-细菌人工染色体转基因动物技术
材料与仪器器材：①离心机②培养箱③水浴锅④小漏斗、滤纸⑤Nucleonond BAC 100 柱⑥Qiagen 大提质粒试剂盒⑦分光光度计⑧脉冲场凝胶电泳（PF
2024-12-21 15 -
通过重组逆转录病毒（慢病毒）载体感染制备转基因动物
简介慢病毒载体是近几年发展起来的新型转基因载体。慢病毒属逆转录病毒的亚科，慢病毒载体经结构改造后，不在宿主细胞内增殖，也不会导致寄主细胞的死亡，受感染动物细胞能
2024-12-21 19 -
基于胚胎干细胞同源重组的基因敲除技术
简介同源重组是遗传重组的一种类型，是指两条相似或相同的 DNA 链之间发生核酸序列的交换而引起 DNA 重新排列。这个过程包括 DNA 的断裂和重新连接等若干步
2024-12-21 19 -
神经组织细胞培养
1． 获取脑组织后，先仔细剥除脑膜和血管等纤维成分，置入 Hanks 液中漂洗 1 ～ 2 次后，置于 30 ～ 50 倍的 Hanks 液中，脑组织比较柔软，
2024-12-21 17 -
Analysis of Testosterone in Serum Using Mass Spectrometry
For either gas chromatography mass spectrometry (GC-MS) or liquid chromatography
2024-12-21 11 -
Differentiation of Neuroepithelia from Human Embryonic Stem Cells
We describe the method for efficiently differentiating human embryonic stem cell
2024-12-21 22 -
Analysis of Male Aggressive and Sexual Behavior in Mice
Pheromone and odor signals play a pivotal role in male mouse reproductive behavi
2024-12-21 12 -
Affinity Processing of Cell-Containing Feeds Using monolithic Macroporous Hydrogels, Cryogels
monolithic macroporous hydrogels, “cryogels,” are produced by polymerization in
2024-12-21 14 -
The Physiology of the Xenopus laevis Ovary
Xenopus laevis has been used for many decades to study oocyte development and ma
2024-12-21 19 -
Adsorption of Lipase on Inorganic Supports
Adsorption is a very economical procedure for immobilization of enzymes. The sta
2024-12-21 15 -
Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template
Oligonucleotide-mediated mutagenesis is a predictable and flexible means of intr
2024-12-21 20 -
Isotype Determination of Monoclonal Antibodies by Immunodiffusion
Immunodiffusion is an important qualitative method for the detection of antigens
2024-12-21 12 -
ORGANOTYPIC KIDNEY CULTURES
ORGANOTYPIC KIDNEY CULTURES-embryos are dissected from timed-pregnant mice from
2024-12-21 16 -
Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids
Acrolein is an α,β-unsaturated aldehyde with enormous capacity of reaction, occu
2024-12-21 13
