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An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs
Antimicrobial peptides (AMPs) play an important role in the host’s innate defenc
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Superparamagnetic Iron Oxide Labeling of Stem Cells for MRI Tracking and Delivery in Cardiovascular
In the mid-1980s, iron oxide nanoparticles were developed as contrast agents for
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Image Cytometry: Nuclear and Chromosomal DNA Quantification
Image cytometry (ICM) associates microscopy, digital image and software technolo
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Aseptic Technique and Good Cell Culture Practice
Aim To ensure all cell culture procedures are performed to a standard that will
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Trafficking of Stem Cells
Stem cells undergo regulated trafficking from the developmental stages to the ad
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Comparison of Molecular Typing Methods Applied to Clostridium difficile
Since the 1980s the epidemiology of Clostridium difficile infection (CDI) has be
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Neurulation and Neural Tube Closure Defects
The purpose of this chapter is to summarize some technical approaches and aspect
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Complexation of siRNA and pDNA with Cationic Liposomes: The Important Aspects in Lipoplex Preparatio
In the last two decades, cationic liposomes have been investigated as vehicles f
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Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos
The development of transgenic technology during recent years has allowed researc
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Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Hum
The successful isolation and ex vivo expansion of resident cardiac stem/progenit
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早产儿视网膜病变动物模型的建立
简介早产婴儿其周边视网膜血管尚未发育,存在着血管未发育区域,是早产儿视网膜病变的发病机制。原理将小鼠放入高氧环境会出现视网膜血管增生类似 ROP 的现象。用途本
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坏死性小肠结膜炎动物模型的建立
简介目前认为早产、缺氧窒息、感染等是 NEC 发病的高危因素。选用新生大鼠,给予根据上述独立危险因索可建立新生儿坏死性小肠结肠炎动物模型。原理根据 NEC 发病
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流式细胞术检测实验-流式细胞术检测胎肝细胞凋亡
材料与仪器器材:① 流式细胞仪管② 手术器械③ 1 mL 注射器④ 流式细胞仪(BDFACSCalibur) 等。试剂:① Annexin V-FITC 细胞凋
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流式细胞术检测实验-流式细胞术检测检测胎肝 Lin-c-Kit+Sca-1+细胞数
材料与仪器器材:① 流式细胞仪管② 手术器械③ 1 mL 注射器④ 流式细胞仪(BD FACSCalibur)。试剂:① e-Kit-APC② Sca-1-FI
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X 线分析实验
简介骨骼是一种从体外看不到的组织,但自从 Whilhelm Röntgen 发现了 X 线后,X 线成为一种使得骨骼疾病可视化的强大技术。X 线具有范围在 10
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