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The Physiology of the Xenopus laevis Ovary
Xenopus laevis has been used for many decades to study oocyte development and ma
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Adsorption of Lipase on Inorganic Supports
Adsorption is a very economical procedure for immobilization of enzymes. The sta
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Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template
Oligonucleotide-mediated mutagenesis is a predictable and flexible means of intr
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Isotype Determination of Monoclonal Antibodies by Immunodiffusion
Immunodiffusion is an important qualitative method for the detection of antigens
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ORGANOTYPIC KIDNEY CULTURES
ORGANOTYPIC KIDNEY CULTURES-embryos are dissected from timed-pregnant mice from
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Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids
Acrolein is an α,β-unsaturated aldehyde with enormous capacity of reaction, occu
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Cloning and Expression Analyses of Pig Genes
Understanding the molecular bases of xenograft rejection is one of the highest p
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Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells
Embryonic stem cells represent a pluripotent population of cells capable of self
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Growth of Human Embryonic Stem Cells in Long-Term Hypoxia
Human embryonic stem cells (hESCs) hold a great promise for regenerative medicin
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Plasma Nitrite and Nitrate Determination
The concentrations of nitrite and nitrate in blood plasma are important in clini
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Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spec
Chromatin is unevenly distributed within the eukaryote nucleus and it contribute
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Determination of Polyamines by Capillary Electrophoresis
The polyamines, mainly putrescine (PU), spermidine (SPD), and spermine (SPM) (Fi
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染色体早熟凝集(premature chromosome condensation,PCC)
将处于分裂期(M期)的细胞与处于细胞周期其他阶段的细胞融合, 使其他期细胞的染色质提早包装成染色体, 这种现象称为染色体早熟凝集(premature chrom
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Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Embryoid bodies (EBs) can be generated by culturing human pluripotent stem cells
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Preparation of cytoplasmic extracts for the application in a cell-free system
Description Cells are grown to 80% confluency, then harvested, washed and disrup
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