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萤光素酶载体PGL3转染细胞到底要不要加G418
相关专题 总有一种转染方法适合你问：最近要做萤光素酶载体PGL3转染细胞，PGL3载体上面带有neo的抗性标记，有一个老师和我说要培养基里面要加G418筛选，但
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Characterization of RNAProtein Interactions by Phosphorothioate Footprinting and Its Applications to
Analogs of naturally occurring substances obtained by chemical modifications are
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Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells
Initial attempts to cultivate mesenchymal stem cells (MSCs) were more successful
2024-12-21 11 -
Imaging the Cytoskeleton in Live Xenopus laevis Embryos
Historically, much of our understanding of actin filaments, microtubules and int
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Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Ro
Mosaic karyotypes are present in at least 0.3–1% of the clinical cases analyzed
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Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel
Phosphorylated polyvinyl alcohol (PVA) gel is nontoxic and can be economically f
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A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery
A useful experimental model to study the pathophysiology of cerebral ischemia wi
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Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons
Sensory neurons in the vomeronasal organ (VNO) are thought to mediate a speciali
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Preparation of RNA Dot-Blots
RNA dot hybridizations were first described by Kafatos et al. (1 ). They enable
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Molecular Imaging and Tracking Stem Cells in Neurosciences
Stem cell transplantation is a promising new therapeutic option in different neu
2024-12-21 13 -
细胞房工作守则
相关专题 一、 常规操作 1. 穿着专用实验服。自己的白大衣或厚外套，可脱在门外的衣帽架上。 2. 穿着专用拖鞋。在缓冲间换下自己的鞋子，尽量避免在缓冲间走动
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PCR反应条件的选择
PCR反应条件为温度、时间和循环次数。 温度与时间的设置： 基于PCR原理三步骤而设置变性-退火-延伸三个温度点。在标准反应中采用三温度点法，双链DNA在90
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dictyBase and the Dicty Stock Center
dictyBase is the model organism database that houses all the sequence and associ
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Freezing and Thawing of Mammalian Cell Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mamm
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Tips for the Functionalization of Nanoparticles with Antibodies
Multiple antibody immobilization methodologies have been developed for several a
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