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Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
Immobilized Metal ion affinity chromatography (IMAC) is a ubiquitous technique i
2024-12-21 12 -
Neural Patterning and CNS Functions of Wnt in Zebrafish
Wnt signaling plays several important roles in the development of the zebrafish
2024-12-21 10 -
Detection of Cells Programmed to Die in Mouse Embryos
Programmed Cell Death (PCD) is a broad term used to describe a series of events
2024-12-21 9 -
Clinical Studies of Antisense Oligonucleotides for Cancer Therapy
Until now, the clinical demise of cancer has relied on surgical resection and th
2024-12-21 11 -
Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation
The early phase of murine embryonic stem (ES) cell differentiation is accompanie
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Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells
Quantification of the expression of individual genes can reveal much concerning
2024-12-21 6 -
Drosophila Cell Lines as Model Systems and as an Experimental Tool
Given the power of Drosophila genetics, it may seem surprising to discover that
2024-12-21 11 -
Quantitation of Transient Gene Expression
In many situations, it is important to quantitate accurately expression of a tra
2024-12-21 9 -
Basics of Flow Cytometry
Flow cytometry is a laser-based technology that is used to measure characteristi
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Quantitative Estimation of Tissue Blood Flow Rate
Tissue blood flow rate (F ) is a critical parameter for assessing functional eff
2024-12-21 11 -
Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin
Langerhans cells (LCs) are the main population of antigen-presenting cells linin
2024-12-21 12 -
Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monola
Increased vascular permeability is the hallmark of inflammation. Here, we descri
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Cloning of the 3 Noncoding Regions from Several Members of Heat Shock Protein Gene Families by Diffe
Several structural and metabolically important proteins in eukaryotes are encode
2024-12-21 12 -
Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryo
Human embryonic stem cells (hESCs) are an invaluable cell source to study human
2024-12-21 8 -
Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients
Septic syndromes represent a major, although largely under-recognized, healthcar
2024-12-21 15
