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Quantification of Protein Carbonylation
Protein carbonylation is the most commonly used measure of oxidative modificatio
2025-01-14 12 -
Plasmid DNA Purification Via the Use of a Dual Affinity Protein
Methods are presented for the production, affinity purification and analysis of
2025-01-14 14 -
RACE实验深度探索：从原理到应用的全面解析
——RACE技术的原理 RACE技术的核心在于利用已知的部分cDNA序列，通过逆转录和PCR扩增，获得完整的cDNA的5′和3′端序列。这一过程分为3′RAC
2025-01-14 17 -
Affinity-Based Assays for the Identification and Quantitative Evaluation of Noncovalent Poly(ADP-Rib
Poly(ADP-ribose) polymerases have been linked to several cellular functions, mos
2025-01-14 15 -
Regulation of MAP Kinase Signaling by Calcium
Mitogen-activated protein kinase (MAPK) signaling influences a variety of cellul
2025-01-14 14 -
Chemokine Receptor Signaling and HIV Infection
The primary function of HIV-1 binding to its chemokine coreceptors is to mediate
2025-01-14 15 -
模板组装(template assembly)
由模板指导,在一系列酶的催化下,合成新的、与模板完全相同的分子。这是细胞内一种极其重要的组装方式, DNA和RNA的分子组装就属于此类。
2025-01-14 18 -
Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors
Protein phosphorylation is a versatile posttranslational modification that can r
2025-01-14 10 -
考马斯亮蓝染色原理
一、染料特性考马斯亮蓝G-250是常用的考马斯亮蓝染料之一。在游离状态下，它通常呈现红色，并且具有两种形态：一种是水溶性的红色形态，另一种是疏水性的蓝色形态。这
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Sequence-Based Typing of HLA: An Improved Group-Specific Full-Length Gene Sequencing Approach
Matching for HLA at the allele level is crucial for stem cell transplantation. T
2025-01-14 13 -
Overview of Cell Synchronization
Widespread interest in cell synchronization is maintained by the studies of cont
2025-01-14 9 -
考马斯亮蓝染色步骤全解析
1）前期准备电泳结束：首先，通过SDS-PAGE电泳将蛋白质进行分离，电泳结束后，取出凝胶。清洗凝胶：将凝胶放入加有超纯水的平皿中润洗，以去除残留的电泳
2025-01-14 15 -
Isolating mRNA and Small Noncoding RNAs from Human Sperm
The isolation of spermatozoal RNA is a challenging procedure due to the intrinsi
2025-01-14 18 -
Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
Cytoplasmic egg extracts from the frog Xenopus laevis represent a powerful cell-
2025-01-14 10 -
考马斯亮蓝测蛋白浓度步骤
1.准备试剂与器材考马斯亮蓝G-250试剂：通常是将100mg考马斯亮蓝G-250溶于50mL的95%乙醇中，再加入适量的85%磷酸，最后用蒸馏水稀释至1L
2025-01-14 16
