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常规组织传代培养
1. 吸除培养瓶内旧培养液。2. 向瓶内加入胰蛋白酶和 EDTA 混合液少许,以能覆满瓶底为限。3. 置温箱中 2~5 分钟,当发现细胞质回缩,细胞间隙增大后,
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Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors
The tachykinins represent the largest known peptide family and are responsible f
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Analysis of Nuclear Export Using Photoactivatable GFP Fusion Proteins and Interspecies Heterokaryons
In this chapter, we review protocols for the analysis of nucleocytoplasmic shutt
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In Vivo Matrigel Migration and Angiogenesis Assays
Angiogenesis, the process of new blood vessels forming from preexisting vessels,
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Vesicular Phospholipid Gels
Highly concentrated phospholipid dispersions of vesicular morphology, Vesicular
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Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Rea
We have had a long term interest in the neuropathogenesis of human immunodeficie
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兔子髓过氧化物酶(MPO)酶联免疫分析(ELISA)
兔子 髓过氧化物酶(MPO) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定兔子血清,血浆及相关
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Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis
Polyacrylamide gel electrophoresis (PAGE) in the presence of the anionic deterge
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Ciona Genetics
Ascidians, such as Ciona , are invertebrate chordates with simple embryonic body
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Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability
Endothelial permeability measurements of intact vascular beds and monolayer cult
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Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Em
Differential gene expression is an essential mechanism for the development of a
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Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo
The examination of the epithelial barrier has been a primary site of focused res
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血清使用过程中应注意的问题和血清质量的决定因素
相关专题 一、关于血清使用的几点建议:1、血清必须贮存-20℃,如存放于4℃,请勿超过两周,对于某些单位一次无法用完一瓶,可在无菌条件下将其40-45ml分装
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小鼠染色体重组技术
简介同源重组(homologous recombination)是遗传重组的一种类型,是指两条相似或相同的 DNA 链之间发生核酸序列的交换而引起 DNA 重新
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鲍曼不动杆菌感染动物模型-甲氨蝶呤免疫抑制小鼠鲍曼不动杆菌感染肺炎动物模型
简介微创微量气管直接注射法建立的感染模型以小鼠死亡为主, 肺部感染模型成功率高。超声雾化法建立的感染模型可以模拟临床感染性肺炎的整个过程, 相似性。 重复性、可
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