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  • The Extraction and Affinity Chromatography of Messenger RNA

    The Extraction and Affinity Chromatography of Messenger RNA

    Living cells contain two forms of nucleic acid-deoxyribonucleic acid (DNA) and r

    2025-01-14 11
  • 考马斯亮蓝染色常见问题解析

    考马斯亮蓝染色常见问题解析

    一、染色不均匀‌问题解析‌:染色不均匀可能是由于样本未固定或清洗干净,染色液浓度不均,染色温度不适宜,或染色时间不够等导致的。‌解决方案‌:①确保样本已经固定并

    2025-01-14 24
  • Methods for Culturing Mouse and Human Embryonic Stem Cells

    Methods for Culturing Mouse and Human Embryonic Stem Cells

    Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 yea

    2025-01-14 14
  • A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of S

    A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of S

    A phenomenological model is proposed for the evaluation of relative electrophore

    2025-01-14 9
  • Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and C

    Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and C

    We describe protocols for the isolation of satellite cells from human muscle bio

    2025-01-14 14
  • Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems

    Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems

    Efficient manipulation of Hedgehog (HH)/GLI signaling activity is crucial to the

    2025-01-14 10
  • 考马斯亮蓝染色液配方 轻松掌握实验技巧

    考马斯亮蓝染色液配方 轻松掌握实验技巧

    ——配方组成‌考马斯亮蓝R250‌:0.5克。考马斯亮蓝R250是主要的染料成分,能够与蛋白质结合并显色。‌异丙醇‌:125毫升。异丙醇作为有机溶剂,有助于染料

    2025-01-14 17
  • Quantification of Cell Cycle-Arresting Proteins

    Quantification of Cell Cycle-Arresting Proteins

    Cellular senescence, which can be defined as a stress response preventing the pr

    2025-01-14 13
  • The Secret Lives of Dictyostelium

    The Secret Lives of Dictyostelium

    Research on Dictyostelium has a long history, the trend of which has been to add

    2025-01-14 15
  • 考马斯亮蓝染色注意事项

    考马斯亮蓝染色注意事项

    一、染色前准备‌①彻底清洁‌:在染色前,务必将待染色的样品(如凝胶、织物等)彻底清洗干净,去除污渍和杂质,以免影响染色效果。‌②选择合适的染色容器‌:确保染色容

    2025-01-14 13
  • TSA多色免疫荧光原理介绍

    TSA多色免疫荧光原理介绍

    一、技术基础TSA技术利用抗原抗体反应对样本中的多种蛋白标志物进行标记。在这一过程中,辣根过氧化酶(HRP)扮演着至关重要的角色,它能够对靶蛋白或核酸进行高密度

    2025-01-14 18
  • TSA多色免疫荧光技术教程

    TSA多色免疫荧光技术教程

    一、实验原理TSA技术利用辣根过氧化酶(HRP)对靶蛋白或核酸进行高密度原位标记。在过氧化氢(H2O2)环境中,非活性的酪胺分子(T)被二抗偶联的HRP催化激活

    2025-01-14 11
  • TSA多色免疫荧光结果解读

    TSA多色免疫荧光结果解读

    一、结果呈现TSA多色免疫荧光实验的结果通常通过显微镜观察获得,表现为不同颜色标记的荧光信号。这些信号代表了样本中不同靶标蛋白的存在和分布。二、颜色与靶标对应关

    2025-01-14 17
  • TSA多色免疫荧光试剂推荐

    TSA多色免疫荧光试剂推荐

    1. PhenoCode肿瘤特征标志物组合即用型试剂盒(PSP)‌推荐理由‌:‌●创新技术‌:采用“5+1”panel设计理念,即5个固定检测靶标加一个用户自选

    2025-01-14 19
  • TSA多色免疫荧光与单色免疫荧光的区别

    TSA多色免疫荧光与单色免疫荧光的区别

    在科研探索的征途中,免疫荧光技术一直是科研人员手中的得力助手。其中,TSA多色免疫荧光与单色免疫荧光作为两种重要的技术方法,各自具有独特的优势和适用范围。今天,

    2025-01-14 12