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The Extraction and Affinity Chromatography of Messenger RNA
Living cells contain two forms of nucleic acid-deoxyribonucleic acid (DNA) and r
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考马斯亮蓝染色常见问题解析
一、染色不均匀问题解析:染色不均匀可能是由于样本未固定或清洗干净,染色液浓度不均,染色温度不适宜,或染色时间不够等导致的。解决方案:①确保样本已经固定并
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Methods for Culturing Mouse and Human Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 yea
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A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of S
A phenomenological model is proposed for the evaluation of relative electrophore
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Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and C
We describe protocols for the isolation of satellite cells from human muscle bio
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Efficient Manipulation of Hedgehog/GLI Signaling Using Retroviral Expression Systems
Efficient manipulation of Hedgehog (HH)/GLI signaling activity is crucial to the
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考马斯亮蓝染色液配方 轻松掌握实验技巧
——配方组成考马斯亮蓝R250:0.5克。考马斯亮蓝R250是主要的染料成分,能够与蛋白质结合并显色。异丙醇:125毫升。异丙醇作为有机溶剂,有助于染料
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Quantification of Cell Cycle-Arresting Proteins
Cellular senescence, which can be defined as a stress response preventing the pr
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The Secret Lives of Dictyostelium
Research on Dictyostelium has a long history, the trend of which has been to add
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考马斯亮蓝染色注意事项
一、染色前准备①彻底清洁:在染色前,务必将待染色的样品(如凝胶、织物等)彻底清洗干净,去除污渍和杂质,以免影响染色效果。②选择合适的染色容器:确保染色容
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TSA多色免疫荧光原理介绍
一、技术基础TSA技术利用抗原抗体反应对样本中的多种蛋白标志物进行标记。在这一过程中,辣根过氧化酶(HRP)扮演着至关重要的角色,它能够对靶蛋白或核酸进行高密度
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TSA多色免疫荧光技术教程
一、实验原理TSA技术利用辣根过氧化酶(HRP)对靶蛋白或核酸进行高密度原位标记。在过氧化氢(H2O2)环境中,非活性的酪胺分子(T)被二抗偶联的HRP催化激活
2025-01-14 11 -
TSA多色免疫荧光结果解读
一、结果呈现TSA多色免疫荧光实验的结果通常通过显微镜观察获得,表现为不同颜色标记的荧光信号。这些信号代表了样本中不同靶标蛋白的存在和分布。二、颜色与靶标对应关
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TSA多色免疫荧光试剂推荐
1. PhenoCode肿瘤特征标志物组合即用型试剂盒(PSP)推荐理由:●创新技术:采用“5+1”panel设计理念,即5个固定检测靶标加一个用户自选
2025-01-14 19 -
TSA多色免疫荧光与单色免疫荧光的区别
在科研探索的征途中,免疫荧光技术一直是科研人员手中的得力助手。其中,TSA多色免疫荧光与单色免疫荧光作为两种重要的技术方法,各自具有独特的优势和适用范围。今天,
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