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RNAi Screening in Cultured Drosophila Cells
RNA interference (RNAi) has become a widely used tool to analyze biological func
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Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporte
Limited understanding of the mechanisms underlying self-renewal and differentiat
2024-12-19 18 -
Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes
Integrins play critical roles in adhesion and migration of T cells during an imm
2024-12-19 18 -
Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridizati
The genus Daphnia has an intriguing reproductive mode of cyclical parthenogenesi
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Structural Studies of Proteins in Solution Using Proton Nuclear Magnetic Resonance
Nuclear magnetic resonance (NMR) spectroscopy has become established in recent y
2024-12-19 18 -
MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides
The diversity of insect neuropeptides coupled with the limitations from the smal
2024-12-19 17 -
Acetylation of Endogenous STAT Proteins
Acetylation of signal transducer and activator of transcription (STAT) proteins
2024-12-19 15 -
CSF Extract Prep for Spindle Assembly
This protocol is essentially as described by Murray (1991), Cell Cycle Extracts.
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受体酪氨酸激酶(receptor tyrosine kinase, RTKs)
RTKs是最大的一类酶联受体, 它既是受体,又是酶, 能够同配体结合,并将靶蛋白的酪氨酸残基磷酸化。所有的RTKs都是由三个部分组成的:含有配体结合位点的细胞外
2024-12-19 18 -
细胞器DNA的荧光显微观察
相关专题 真核细胞内存在多种细胞器 ，如线粒体、质体、高尔基体、溶酶体和内质网。其中线粒体和质体携带自身的DNA。它们编码着线粒体和质体执行功能所必需的部分R
2024-12-19 17 -
人可溶性细胞间粘附分子3(sICAM-3)酶联免疫分析（ELISA）
人可溶性细胞间粘附分子3(sICAM-3) 酶联免疫分析（ ELISA ）试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆
2024-12-19 15 -
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
Ultraviolet (UV)-dependent photochemical crosslinking is a powerful approach tha
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细胞的原代和传代培养
相关专题 一、原代细胞培养(一)原理 细胞培养(Cell culture)是模拟机体内生理条件，将细胞从机体中取出，在人工条件下使其生存、生长、繁殖和传代，
2024-12-19 18 -
LC3 and Autophagy
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein wi
2024-12-19 22 -
T Cell Culture for Gammaretroviral Transfer
Gene transfer into mature T cells with gammaretroviral vectors requires prestimu
2024-12-19 19
