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Recovery, Isolation, Identification, and Preparation of Spermatozoa from Human Testis
In some cases, human spermatozoa to be used for in vitro fertilization are proce
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Derivation of Contractile Smooth Muscle Cells from Embryonic Stem Cells
Smooth muscle cells (SMCs) play a key role in vascular physiology and pathology.
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Separation of Dna by Capillary Electrophoresis
This chapter reports an overview of the analytical techniques used to perform ge
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Serum-Free Media for Neural Cell Cultures: Adult and Embryonic
The ability to grow primary neurons under serum-free conditions is facilitating
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Differentiation and Dynamic Analysis of Primitive Vessels from Embryonic Stem Cells
Embryonic stem (ES) cells, which are derived from developing mouse blastocysts,
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Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary
The technique of single muscle-fiber cultures has already proven valuable in ext
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Combinatorial Stem Cell Mobilization in Animal Models
It has long been recognized that single therapies, such as G-CSF, have a limited
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Noninvasive Metabolic Assessment of Single Cells
Because metabolism is fundamental to the successful functioning of a cell, pertu
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High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-D
High performance liquid chromatography/electron spin resonance/mass spectrometry
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Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1 or Tumor Necrosis Facto
Hematopoiesis is the process of blood cell formation in the adult human, the bon
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Identification of Oocyte Progenitor Cells in the Zebrafish Ovary
Zebrafish breed year round and females are capable of producing thousands of egg
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Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
The sperm acrosome reaction (AR) is an exocytotic event that involves multiple f
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Laser Microdissection Microscopy: Application to Cell Culture
Laser microdissection (LMD) microscopy allows isolation of specific cell populat
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Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes
A powerful, yet often underutilized tool available to probe the structure of mac
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Liposomal Reconstitution of Monotopic Integral Membrane Proteins
In spite of considerable progress in the methodology for reconstitution of membr
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