-
An Improved Rapid Method of Isolating RNA from Cultured Cells
The purification of good-quality RNA from tissue-cultured cells is essential for
2024-12-20 27 -
Cell-Cell Interactions on Solid Matrices
Models to study molecular, biochemical, and functional responses in vitro genera
2024-12-20 25 -
Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method
Oligonucleotide-directed mutagenesis has become the primary method for testing t
2024-12-20 29 -
Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability
Vascular Endothelial Growth Factor-A (VEGF-A) is one of the most important regul
2024-12-20 24 -
Duplex Unwinding and RNP Remodeling With RNA Helicases
RNA helicases are essential for the adenosine 5′-triphosphate (ATP)-driven rearr
2024-12-20 28 -
In Vitro Delivery of Drugs and Other Molecules to Cells
The permeability of a cell membrane can be transiently increased locally when an
2024-12-20 32 -
Histone Modification Profiling in Normal and Transformed Human Embryonic Stem Cells Using Micro Chro
Comparing normal human embryonic stem cells (hESCs) to those that have acquired
2024-12-20 27 -
Glutaraldehyde-Mediated Protein Immobilization
In this chapter, we describe different approaches for the utilization of glutara
2024-12-20 16 -
Reprogramming of Liver to Pancreas
Islet grafts have demonstrated that patients with diabetes would benefit greatly
2024-12-20 27 -
Assays for Hypermethylation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP
The breast cancer 1 and 2 , early onset (BRCA1 and BRCA2 ) genes are important f
2024-12-20 27 -
Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo
Introducing genes into cells by retroviral transduction has greatly increased th
2024-12-20 26 -
Preparation of tubulin
Although many protocols for tubulin preparation are available, the procedure des
2024-12-20 23 -
Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
Fluorescence live microscopy is a powerful technique to study complex cellular d
2024-12-20 29 -
Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequen
Life expectancy has always been associated to several determinants, such as envi
2024-12-20 24 -
Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatograph
Part A. Sample and Standards Preparation In Chapter 18 , the extraction procedur
2024-12-20 23
