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PCR-SSCP原理和操作步骤
原理PCR-SSCP是1989年日本Orita等创建的筛查突变的新技术。它是一种简单、快速、经济的点突变筛查手段。PCR-SSCP技术的基本原理是PCR扩增后的
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内切酶在PCR反应产物中的活性(Activity of Restriction Enzymes in a Primer Extensio
通常PCR反应以后还要对产物进行酶切才能进入下一步工作。为了方便起见，我们可以将内切酶直接加入到PCR反应产物中去，而省略了纯化产物的步骤。下表归纳了在PCR产
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RT-PCR Analysis--详细的RT-PCR方法
Solutions10X RT Buffer10X PCR Buffer100 mM Tris pH 9.0500 mM KCl1% Triton X-1002
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RT-PCR: The Basics
RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive t
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What is the highest temperature 
QuestionWhat is the highest temperature that SUPERSCRIPT II, MMLV, or THERMOSCRI
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Real-Time or Kinetic PCR
The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied B
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PCR Amplification from Microbial Colonies
RAPD PCR Colony MiniprepEdit the program or pick up a program (No 10) if the pro
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Salt (KCl) concentration
For the successful PCR or multiplex PCR amplification of many loci (especiall
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Polymerase Chain Reaction
Materials:Supplies: Procedures: Results: 上一篇：PCR Amplification of DNA 下一篇：Stan
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Calculating Concentrations for PCR
a) Primers :i) Oligonucleotide primers are generally supplied as "so many O
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PURIFICATION OF PCR PRODUCTS WITH SEPHAD
Place the sephadex measuring plate (MultiScreen ?Column Loader) on a clean piece
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Primer amount in PCR
Absolute value of primer concentration in multiplex PCR . The amount of DNA pri
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Preparation of G+A Marker
Preparation of G+A Marker Author: Long-Cheng Li Source: Protocol Online
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RT-PCR --- one-step
This protocol uses C. therm. Polymerase One-Step RT-PCR system from Roche.1. Set
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PCR with expand Polymerase
Expand High Fidelity PCR (Boehringer)Steve HahnLast modified 12/12/99Expand is a
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