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  • is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea

    The gene we are interested in looking at is not very abundant. At 100 ng/ul, the

    2024-11-19 20
  • SDS-PAGE--聚丙烯凝胶电泳

    SDS-PAGE--聚丙烯凝胶电泳

    DS-PAGE: gel electrophoresis of proteins TECHNIQUE is to set up gel plates befor

    2024-11-19 23
  • General PCR methods

    General PCR methods

    Polymerase Chain Reaction (PCR)(adapted from Bruce A. Roe, Department of Chemist

    2024-11-19 22
  • real-time PCR-Molecular Biology

    real-time PCR-Molecular Biology

    hi,I am going to do real-time PCR using Smart Cycler, but I have neverdone this

    2024-11-19 19
  • Long-PCR Reagents and Guidelines

    Long-PCR Reagents and Guidelines

    Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al.

    2024-11-19 21
  • Isolation of Retroelement from Plant Genomic DNA

    Isolation of Retroelement from Plant Genomic DNA

    The amplification of DNA fragments using the polymerase chain reaction (33) is

    2024-11-19 20
  • PCR基本实验方法(三)

    PCR基本实验方法(三)

    "Hot Start" PCR: In certain circumstances one wishes to avoid mixing p

    2024-11-19 23
  • Polymerase Chain Reaction

    Polymerase Chain Reaction

    Materials:Taq (or other) PCR enzyme (5 Units/ul)   PCR enzyme reaction buffer  

    2024-11-19 21
  • PCR principles and practices

    PCR principles and practices

    Important parameters in the PCR:1.Template DNA quantity (complexity determines n

    2024-11-19 19
  • PCR基本实验方法(四)

    PCR基本实验方法(四)

    Labelling PCR Products with DigoxigeninPCR products may be very conveniently lab

    2024-11-19 18
  • Primer4.10中文使用说明

    Primer4.10中文使用说明

    Primer4.10中文使用说明Primer Premier4.0是由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计,评估的软件,和

    2024-11-19 22
  • Reaction volume

    Reaction volume

    Q: Does the PCR reaction volume (negatively) influence the outcome?A: No, especi

    2024-11-19 24
  • CORE SAMPLE PCR

    CORE SAMPLE PCR

    A method to re-PCR unique bands from products of mixed sizeINTRODUCTIONThe produ

    2024-11-19 25
  • contamination in real-time pcr negative control-Real-Tim

    contamination in real-time pcr negative control-Real-Tim

    hi there,i am noew constantly getting a late peak (30+ cycles) in the negative c

    2024-11-19 22
  • Small amounts of RNA for qRT-PCR-Real-Time PCR

    Small amounts of RNA for qRT-PCR-Real-Time PCR

    I have a quick question. In our lab we have had a great deal of sucess using qRT

    2024-11-19 25