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双向凝胶电泳分析小鼠子宫内膜胚泡着床点和着床旁组织蛋白质组
摘要目的：研究小鼠子宫内膜胚泡着床点和着床旁蛋白质表达图谱及其差异。 方法：用固相pH 梯度双向凝胶电泳分离5d. p. c. （days postcoitum
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猪免疫球蛋白G2a(IgG2a)酶联免疫分析（ELISA）
猪 免疫球蛋白G 2a(IgG2a) 酶联免疫 分析（ ELISA ）试剂 盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定猪血清，血浆及相关液体样本中
2024-12-04 31 -
Western Blot详解－主要试剂
主要试剂 1、 丙烯酰胺和N，N’-亚甲双丙烯酰胺，应以温热(以利于溶解双丙稀酰胺)的去离子水配制含有29%(w/v)丙稀酰胺和1%(w/v)N，N’-亚甲双丙
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Western 不同转膜方法的取舍
转膜: 小分子量的蛋白半干转效果比较好， 大分子量（100KD）以上建议湿转。 具体转膜条件需要多摸一下。30-80KD半干转恒流1mA/cm2，1-1.5h
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丙烯酰胺胶中肽提取和蛋白消化技术Digestion of Proteins and Extraction of Peptides from an Acrylamide
Digestion of Proteins and Extraction of Peptides from an Acrylamide GelSherry Ni
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Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-B
2024-12-04 25 -
蛋白质组学-质谱分析
Proteomics Primer 1. Proteomics 2. 2-D PAGE 3. Immobilised pH gradients (IPGs) 4
2024-12-04 26 -
Preparation of Brain Membrane Fractions for Western Blot Analysis
Preparation of Brain Membrane Fractions for Western Blot AnalysisAll the procedu
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Western Blot
Western Blot1) Run protein gel.Set up blot: 2) Prepare two tupperware containers
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Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots The follow
2024-12-04 21 -
美国实验室wetern方法
WESTERN BLOT PROTOCOL In a Western blot, proteins that are separated on polyacry
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Affinity chromatography (with HIS-tagged proteins)
Affinity chromatography (with HIS-tagged proteins)This protocol is described in
2024-12-04 22 -
WESTERN PROTOCOL
WESTERN PROTOCOL A. Preparation of cell lysatesCollect cells (confluent T-25) by
2024-12-04 21 -
蛋白酶抑制剂资料 Protease Inhibitors
Protease Inhibitors from Alberts' Lab Protocols (Kathy Miller/Chris Field)
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HISTONE KINASE ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western bl
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