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Protocol for First-strand cDNA Synth
Prepare in a sterile tube:template RNA: total RNA 0.1-5µg or poly(A)+ mRNA 10n
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Use of Internaland External Standardsor Reference RNAs for A
IntroductionNorthern blotting, nuclease protection assays and RT-PCR are frequen
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S1analysis of yeast mRNA using oligo nucleotide probes
Steve Hahn. last modified Sat, Oct 17, 1998Mix the following in an 0.5 ml eppend
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RNA purification --- hot phenol
Hot phenol may also be used to remove DNA.1. Add equal volume of TE-saturated ph
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ESTs定义
ESTs（Expressed Sequence tags）是从已建好的cDNA库中随机取出一个克隆，从5’末端或3’末端对插入的cDNA进行一轮单向自动测序，所
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Polymerase III in vitro Transcriptio
Polymerase III in vitro TranscriptionSteve Hahnlast modified 10/15/99For the fol
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转录分析的几种方法
信号通路，只有一个目的：将细胞的外部信号转换成细胞的内部变化。不管是胰岛素，还是异亮氨酸，抗原还是肾上腺素，它们的终极目的总是如出一则：对转录活性的改变。这些对
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In vitro RNA synthesis from plasmid-borne sequences under th
N.B: Gloves should be worn at all times during preparation of in vitro RNA. All
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培养细胞RNA提取--RNA Isolation Protocol-Cultured Cell Samples
Required Reagents: Chloroform Isopropanol 75% Ethanol (cold) Monophasic Isolatio
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相似性序列发生Cross－react引起脱靶效应
监测手段：对脱靶效应的监测一般通过基因芯片来监测。Gene expression profiling对脱靶效应。 脱靶效应的特性： 转录物表达模式有序列特异性和
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研究揭示miRNA调控新途径
自荷兰癌症研究院（The Netherlands Cancer Institute）肿瘤生物学部、德国马克斯·普朗克生物物理化学研究院（Max-Planc
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Primer Extension analysis of total yeast RNA
Primer Extension analysis of total yeast RNA1. Mix the following in a 1.5 ml epp
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DIETHYL PYROCARBONATE (DEPC) TREATMENT OF AQUEOUS SOLUTIONS
Diethyl pyrocarbonate derivitizes histidine residues and is therefore an effecti
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3'RACE PCR
3'RACE PCR 3' R apid A mplification of c DNA E nds (RACE ) PCRThis techn
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RNase Protection Assay
The ribonuclease protection assay (RPA) is a highly sensitive and specific metho
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