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Invitro transcription
The following protocol is for MEGAscript II Kit (Ambion).1. PCR amplify the DNA
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Total RNA extraction from Arabidopsis and tobacco--拟南芥和
RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercapto
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The Easiest Route to Guaranteed
The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are u
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Background Information of RNAi
Background Information of RNA iRNA interference (RNA i) is a biological process
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什么是RNA interference（RNAi）
RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximat
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RNAi效果的测量
检测基因特异阻断的最常用的方法是进行westernblot分析，比较导入siRNA 前后蛋白表达水平的变化。在一些情况下可能使用检测报告子基因的报告子系统例如β
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The Easiest Route to Guaranteed
The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are u
2024-11-06 17 -
RNAi术语表
RNA i GlossaryDicer - Dicer is a member of the RNase III family of nucleases tha
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In vitro transcription reaction
1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X tra
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RNAi在细胞培养中的应用
The protocols listed here are for Drosophila cells in 6 well plates and our pre-
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RNA干扰在人类T细胞的应用
RNA干扰在人类T细胞的应用在2006年3月24日的Journal of Immunological Methods杂志上，来自费城儿童医院的一个研究组使
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PolymeraseIII invitro Transcription
Polymerase III in vitro TranscriptionSteve HahnFor the following reactions, use
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活体RNAi关键点
在活体RNAi关键点：类似药物属性的引入，如稳定性、细胞内的传送、组织的生物可溶性进入合成的siRNA. 授予siRNA类似药物的属性 化学稳定性的胆固醇共聚的
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HeTube Mating with MSCV recipient
Chemicals needed:GlucoseL-( )-Arabinose Sigma Cat # A3256L-Rhamnose Sigma cat #
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提取棉花RNA--RNAEXTRACTION METHOD FOR COTTON
Based on: Wan, C.-Y. and Wilkins, T.A. 1994. Anal. Bioch. 223:7-12.1.Collect you
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