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  • Agarose-Formaldehyde Electrophoresis

    Agarose-Formaldehyde Electrophoresis

    Agarose-Formaldehyde ElectrophoresisRNA electrophoresis under denaturing conditi

    2024-11-02 14
  • Protein-DNA Telomere FISH

    Protein-DNA Telomere FISH

    Protein-DNA Telomere FISH FIX AND PROTEIN STRAIN:Fix cells in 4% paraformaldhyde

    2024-11-02 20
  • Northern Blotting: Efficient RNA Staining and Transfer

    Northern Blotting: Efficient RNA Staining and Transfer

    (original protocol by R. M. Fourney, S. Miyakoshi, R. S. Day III, and M. C. Pete

    2024-11-02 17
  • Pre-hyb/hyb for Radioactive Probes

    Pre-hyb/hyb for Radioactive Probes

    Pre-hyb/hyb for Radioactive ProbesStuff you need:50X Denhardt's reagent:1% F

    2024-11-02 19
  • RNAi-制备siRNAs的方法

    RNAi-制备siRNAs的方法

    越来越多的研究人员开始采用小分子干扰RNA(small interfering RNAs,siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链

    2024-11-02 23
  • The Neverfail Northern Blot Hybridization

    The Neverfail Northern Blot Hybridization

    Prehybridization/Hybridization Solution 0.1% SDS 50% Formamide 5x SSC 50 mM NaP

    2024-11-02 18
  • E.coli Total RNA Labeling Protocol for Spotted Microarray

    E.coli Total RNA Labeling Protocol for Spotted Microarray

    Note: Start with 20 mg of total RNA for each labeling reaction. All solutions th

    2024-11-02 16
  • Hiro Hirai PRINS Protocol

    Hiro Hirai PRINS Protocol

    Hiro Hirai 's PRINS ProtocolOverviewThe PRINS (Primed in situ ) technique us

    2024-11-02 15
  • [Nature] RNAi研究专题

    [Nature] RNAi研究专题

    Cells utilize RNA interference (RNAi) to regulate protein expression in many con

    2024-11-02 21
  • Primer Extension analysis of total yeast RNA

    Primer Extension analysis of total yeast RNA

    Primer Extension analysis of total yeast RNA1. Mix the following in a 1.5 ml epp

    2024-11-02 18
  • Northern Blotting (Howell Lab)

    Northern Blotting (Howell Lab)

    RNA isolationHarvest cells when they are about 80-85% confluent. Stationary phas

    2024-11-02 20
  • RNA干扰沉默癌基因

    RNA干扰沉默癌基因

    RNA interference silences cancer geneDavid Douglas Reuters Health Posting Date:

    2024-11-02 26
  • Collection of Tissue for RNA Analysis

    Collection of Tissue for RNA Analysis

    Handling RNA: Ribonucleases (RNases) are very stable and active enzymes that deg

    2024-11-02 13
  • Decontamination and inhibition of ribonucleases

    Decontamination and inhibition of ribonucleases

    Decontamination and inhibition of ribonucleasesIt is common knowledge in most la

    2024-11-02 18
  • 用异硫氰酸胍和有机溶剂提取RNA

    用异硫氰酸胍和有机溶剂提取RNA

    收集细胞,离心5000r/min ,5分钟,弃上清,加入异硫氰酸胍变性液(异硫氰酸胍4mol/L;柠檬酸钠25mmol/L,Sarkosy10.5%,β-巯基乙

    2024-11-02 15