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用异硫氰酸胍和有机溶剂提取RNA
收集细胞，离心5000r/min ，5分钟，弃上清，加入异硫氰酸胍变性液（异硫氰酸胍4mol/L；柠檬酸钠25mmol/L，Sarkosy10.5%,β-巯基乙
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C.elegans TOTAL RNA PREP
1. Collected animals can be stored as frozen pellets at -70 degrees. Alternativ
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五种不同的制备siRNA方法的比较
比较项目化学合成体外转录RNase III降解dsRNAsiRNA 表达载体PCR 表达框材料需求21-mer RNA oligos(一对)29-mer DNA
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RNA干扰技术的新突破
来自一家名为“ＡＬＮＹＬＡＭ”的 生物 技术公司的研究人员在11日出版的《自然》杂志上报告说，他们通过转基因技术在ＲＮＡ（核糖核酸）干扰技术的研究上取得了突破，
2024-11-02 20 -
Northern杂交试验指导手册－模板DNA的制备
A．质粒DNA的小量提取 试剂及其配制 含AP的LB液体培养基 TE缓冲液：10mmol/L Tris-HCl (pH 8.0) 1mmol/L EDTA (p
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miRNA的特征、功能及识别方法等详解
什么是miRNA MicroRNAs （miRNAs） 是一种大小约21—23 个碱基的单链小分子RNA ，是由具有发夹结构的约70-90 个碱基大小的
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Northern transfer protocol
A. Formaldehyde agarose gel electrophoresisNote: Formaldehyde vapours are toxic
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Fluorescence In Situ Hybridization
PRE-TREATMENTFix the cells with Carnoy’s and drop them onto a glass slide Air dr
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Hiro Hirai BAC-FISH Protocol
Hiro Hirai 's BAC-FISH ProtocolOverviewThis protocol is a modification of th
2024-11-02 19 -
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
Abstract: This is the standard and complete Northern blotting protocol including
2024-11-02 14 -
Northern blot实验技术
一、经乙二醛和二甲基亚砜变性处理后进行的RNA电泳这一方法原于McMaster和Carmichael(1977)。 含有乙二醛－DMSO的凝胶比含有甲醛的凝胶更
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RNA Isolation Protocol from LCM Samples
1. Drop sample into 100 μl Lysis Solution and incubate for 30 min at 42CUsing sh
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Protocol of Northern blot
Protocol of Northern blot 质粒的转化和扩增 质粒的鉴定 目的基因片段的切割 3.1样品双酶切(175μl水解体系) DW 115μl
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Hiro Hirai PRINS Protocol
OverviewThe PRINS (Primed in situ) technique uses a specific primer, dNTPs with
2024-11-02 23 -
Preparation of Poly A+ RNA and Northern Analysis
Preparation of Poly A+ RNA and Northern Analysis Procedure (Pharmacia #27-9255-0
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