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粘性末端的连接
1) The ligation mixture contains the following:vector DNA (~100 ng) insert DNA (
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CRISPR技术分享
近年来，CRISPR技术掀起了基因组编辑的热潮。短短一年间，CRISPR技术已登上了数个知名榜单，包括Science的十大科学突破，Nature Methods
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T-RFLP简介
T-RFLP中文可翻译为：末端限制性片段长度多样性，又可以称为16sRNA基因的末端限制性片段分析技术。是一种新兴的研究微生物多态性的分子生物学方法，日亦受到研
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CsClPrepofPlasmidDNA
This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1
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Isolation of DNA from Acrylamide GELS
1. Pour a vertical acrylamide gel using TEA buffer. A 4 % non denaturing gel is
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DNAandRNAEXTRACTIONS
1) Take one medium sized leaf or half a large leaf (5 to 20 cm^2), weigh and fre
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CAT ASSAY (liquid phase)
1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour into
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Phenol chloro form Extraction of DNA
Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturat
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Tail Preps: DNA Isolation From
NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR.(from Ruixia, 7/9
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DNAPurificationfromAgaroseGels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide
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OligonucleotidePurification
Purpose: Purification of oligonucleotides (which have already been purified by r
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Plasmid Quickpreps
1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin
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BUCCAL CELL DNA PREPS
Important : Extract the DNA within one week of receiving samples. Samples that h
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NH4Ac and EtOH precipitation of DNA
NH4 Ac and EtOH precipitation of DNAAdd NH4 Ac (10M stock or solid) to the sampl
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Alternate Method for Purifying DNA
1.Excise band of interest from a TAE gel; estimate volume by weighing the gel sl
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