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DNAPurificationbyphenolextractionandethanolprecipitation
Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM E
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Isolating DNA Fragments
Materials:• 0.8 % agarose gel in 1x TAE• Digested DNA• Glass Milk
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Target enrich mentte chnologies for ABISOLiD
Q:my group is involved in a national project to develop a genomic platform for c
2024-10-21 16 -
Ribonuclease Protection Assay
1. Add appropriate volumes of RNA (<12 ul) plus probe (< 3 ul). Try 5 and
2024-10-21 10 -
Self-circularization of Linear DNA
一、In a microcentrifuge tube prepare a solution of linear DNA (25-50ng) in deioni
2024-10-21 11 -
Electro-elutionofnucleicacidsfromagaroseandpolyacrylamidegelslides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed ou
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DNAISOLATIONFROMPRIMARYTUMORSVIACRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
2024-10-21 12 -
Improved Alcohol Precipitation of DNA
ECKDescriptionThis method was suggested in a BRL "Focus" article sever
2024-10-21 10 -
DNA Fragment Purification from Agarose or Acrylamide
DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to
2024-10-21 14 -
Preparation of GA Marker
Author: Long-Cheng LiSource: Protocol OnlineAbstract: Simplified method for prep
2024-10-21 12 -
RecoveringDNAfromagarosegels
IntroductionMethods and reagents is a unique monthly column that highlights curr
2024-10-21 10 -
Boilinglysismini-prep
1、Grow an overnight culture from a single colony in 2 mls LB antibiotic.2、Transf
2024-10-21 12 -
TACloning
TA CloningI. Initial mixture5 μl dd H2O1 μl PCR product1 μl ligation bu
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NH4AcandEtOHprecipitationofDNA
Add NH4Ac (10 M stock or solid) to the sample for a final concentration of 2.5 M
2024-10-21 12 -
ElectroelutionofDNAfromAgarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 ml
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