-
如何来完成mRNA的纯化？
mRNA的分离方法较多，其中以寡聚（dT）-纤维素柱层析法最为有效，已成为常规方法。此法利用mRNA 3’末端含有Poly（A+）的特点，在RNA流经寡聚（dT
2024-10-21 12 -
Practical Tips for Optimal Northern Analysis
Northern assays require RNA (total- or poly(A)- selected) to be resolved on a ag
2024-10-21 14 -
CTAB Procedure
In the hood:96 well dish with bacteriatitertechmicrotubesglass pipetteRemove col
2024-10-21 12 -
Genomic (Plant) DNA: Restriction Digestion for Sou
Background on the art of getting beautiful Southerns:There are several things to
2024-10-21 11 -
Tail Preps: DNA Isolation From
NOTE: THIS IS FINE FOR SOUTHERNS, BUT NOT FOR SCREENING BY PCR.(from Ruixia, 7/9
2024-10-21 16 -
Filterbindingassay简介、原理和方法
Filterbindingassay简介、原理和方法关键词： Filterbindingassay 原理 方法来源： 互联网简介优点：操作简单方便缺陷：不能像E
2024-10-21 13 -
ES CELL DNA EXTRACTION: TUBE ME
Protocol for extracting DNA from ES Cells, starting from the 96-well plate but p
2024-10-21 19 -
DNA From Whole Blood for PCR
1.Obtain 65-100 µl of blood by retro-orbital bleed with a heparinized microcapil
2024-10-21 21 -
Plant Total DNA Isolation
OverviewProcedure1. Preheat the CTAB Isolation Buffer at 60℃.2. Grind 2 g of fre
2024-10-21 14 -
Southern Blot Protocol
Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 ul of 10 ug
2024-10-21 17 -
Largescale nuclear extract preparation
Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Kari
2024-10-21 18 -
UV Quantitation of DNA
DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus
2024-10-21 19 -
DNAPrecipitation
Phenol (removes protein)1.add equal volume of Phenol (= tris-saturated Phenol-Ch
2024-10-21 8 -
Protocols for ET recombination
1、Oligo design（1）The 5' end (the homology arm) - choose 42 or more (we usual
2024-10-21 9 -
Isopropanol precipitation-best time and temperature-M
Hi, does anyone know what is the best temperature and time for Isoprop precipita
2024-10-21 7
