-
DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
2024-10-21 9 -
Preparation of BAC DNA
The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi
2024-10-21 9 -
SimplifiedDNAExtractionfromCellorTissue
PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. L
2024-10-21 13 -
Tg 008 Southern Blotting
Transgenic Mouse and Gene Targeting FacilityTg 008 Southern BlottingMaterials an
2024-10-21 16 -
DNA Purification from Agarose Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide
2024-10-21 10 -
Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 8 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-21 17 -
Preparation of TonsilLysate
PurposeThe protocol describes how to prepare human tonsil lysate for use in puri
2024-10-21 10 -
Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 12 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-21 10 -
Preparation of plasmid DNA（质粒制备）
Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation pr
2024-10-21 13 -
Analysis of Differential Gene Expression
Microarray analysis and differential display have become popular techniques for
2024-10-19 12 -
MINI PREP BY BOILING
Pellet 1.5 ml of an overnight culture at 12,000 rpm in Eppendorf centrifuge at R
2024-10-19 14 -
Phenol/Chloroform Precipitation of DNA
1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake,
2024-10-19 14 -
核酸抽提mRNA的分离技巧
mRNA的分离 与rRNA和tRNA不同的是，哺乳动物细胞的绝大部分mRNA在其3'端均有一poly(A)尾，因此可以用oligo(dT)－纤维素亲和层
2024-10-19 12
