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DNA Purification and Precipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline
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DNAExtractionProtocolsUsingSilica
Below we present the protocols we have used to isolate DNA from various tissues
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CLONING FROM LOW MELT AGAROSE
competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformat
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Isolation of DNA from Agarose Gels(Paper Slurry Method)
Isolation of DNA from Agarose Gels (Paper Slurry Method)This procedure isolates
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DNA Fragment Purification from Agaro
DNA Fragment Purification from Agarose or AcrylamideFor fragments from 200 bp to
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DNA Extraction from Archival Formali
DNA Extraction from Archival Formalin-fixed, Paraffin-embed allowfullscreen='
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Rapid elution of DNA from agarose gels
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, N
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Rapid elution of DNA from agaro
Rapid elution of DNA from agarose gelsJames Movius, Hahn LabLast modified Sun, N
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Yeast boiling DNA miniprep
Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992)Ha
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Aninexpen sivealter native to glass milk for DNA purificatio
Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2.
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Purification of DNA from lambda phage
This is a modification of the procedure in Short Protocols (1-41,1-45)1.Prepare
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Oligonucleotide Purification
Purpose: Purification of oligonucleotides (which have already been purified by r
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Plasmid Quickpreps
1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin
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Preparation of linear polyacrylamide
Preparation of linear polyacrylamide used as carrier in ethanol precipitation of
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OLIGO PURIFICATION on acrylamide gels
GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1.
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