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Template Preparation
The quality of sequencing results is directlyrelated to the quality of the templ
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Southern Blot
1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns, digest
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Plasmid DNA Isolation from Bacteria
Materials:1.TENS solution:10 mM Tris (pH to 7.5)1 mM ethylenediaminetetraacetic
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MITOCHONDRIAL DNA ISOLATION
ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A
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DNA Plasmid Maxiprep Protocol
1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampi
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Preparation of Sonicated Salmon Sper
Preparation of Sonicated Salmon Sperm DNAProcedure:1) Use Pharmacia #27-4564-01.
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CAT ASSAY (liquid phase)
1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks, shake & pour into
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RNase Protection Assay (Beverly Faulkner-Jones)
RNase Protection Assay - ProtocolThis method can be used to detect and quantitat
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BAC DNA Isolation with AutoGen 740
1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB
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DNA FRAGMENT PURIFICATION W/ GLASS WOOL
Glass wool method:Run TAE agarose gel and cut the appropriate band out with a cl
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DNA Precipitation
Phenol (removes protein)1、add equal volume of Phenol (= tris-saturated Phenol-Ch
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Recovery of DNA from LMP (Low Melting Point) Agarose Gel
Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragment
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Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydro
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DNA Purification by phenol extraction and ethanol precipitation
Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM
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Mini DNA extraction from wheat leaves for PCR and Southern B
Procedure for 100 ~ 300 mg of tissue.1. Collect fresh tissue, coleoptiles or sma
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