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UV Quantitation of DNA
DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus
2024-10-17 21 -
NH4Ac and EtOH precipitation
NH4Ac and EtOH precipitation of DNAAdd NH4Ac (10M stock or solid) to the sample
2024-10-17 17 -
Mag-Bind Soil DNA Kit Protoco
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sam
2024-10-17 15 -
Boiling lysis mini-prep
1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Tran
2024-10-17 17 -
Concentration of DNA by ethanol precipitation
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Univer
2024-10-17 18 -
Purification of PCR fragments for cloning
After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder
2024-10-17 20 -
DNA Plasmid Miniprep Protocol
1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicill
2024-10-17 16 -
DNA extraction from blister rust aeciospores and urediniospores using dry grindi
Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Mole
2024-10-17 16 -
Diatomaceous Earth-based Midi-prep
Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice
2024-10-17 13 -
Genome Walking Kit 使用注意
用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.
2024-10-17 13 -
Increasing Sensitivity in Northern Analysis with RNA Probes
Note: The pointers in this technical bulletin presuppose the use of standard 50%
2024-10-17 12 -
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-gluc
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PROTOCOL TO EXTRACT DNA FROM PA
PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of formali
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MCA
2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polymeras
2024-10-17 16 -
S1 Nuclease Protection Assay
End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci
2024-10-17 20
