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  • RNA Extraction from Frozen Tissue Sections

    RNA Extraction from Frozen Tissue Sections

    Tissue Handling: Note that all unfixed human tissue should be handled as BioSafe

    2024-09-24 22
  • 基因治疗-- 基因治疗的策略

    基因治疗-- 基因治疗的策略

    第十四章 基因治疗  基因治疗(gene therapy)是指将外源正常基因导入靶细胞,以纠正或补偿因基因缺陷和异常引起的疾病,以达到治疗目的。也就是将外源基因

    2024-09-24 22
  • 全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fi

    全基因组的比较基因组杂交技术介绍(Whole-Genome and Custom Fi

    Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (

    2024-09-24 17
  • DNA methyltransferase Assay

    DNA methyltransferase Assay

    Methylated CpG island Amplification Protocol written by Minoru Toyota2. Material

    2024-09-24 29
  • 多基因病

    多基因病

    第二节 多基因病一些常见的先天畸形(congenital maiformation)和常见而病因复杂的疾病,其发病率一般都超过1/1000,疾病的发生都有一定的

    2024-09-24 22
  • Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)

    Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)

    This protocol is based upon protocols from Mark Biggin, Dave Allis and Richar

    2024-09-24 23
  • Transgenic Mouse and Transgenic Rat Outline

    Transgenic Mouse and Transgenic Rat Outline

    1. Introduction. This is a brief outline of the steps necessary to obtain transg

    2024-09-24 18
  • Nick Translation of DNA for CGH

    Nick Translation of DNA for CGH

    1. Prepare reaction mixtures per 50ul Add Enzymes last, gently vortex mixture, q

    2024-09-24 22
  • Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana

    Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana

    IntroductionEukaryotic chromatin is a complex of DNA and associated histone prot

    2024-09-24 28
  • 基因的复制与表达

    基因的复制与表达

    第三节 基因的复制与表达生物的遗传物质基础是核酸(nucleic acid),它也是基因的基本结构,它们的化学组成分子结构符合遗传物质的稳定性、连续性及多样性的

    2024-09-24 25
  • In Planta Transformation of Arabidopsis

    In Planta Transformation of Arabidopsis

    In Planta Transformation of ArabidopsisDetlef Weigel and Jane GlazebrookThis pro

    2024-09-24 24
  • DNA甲基化分析

    DNA甲基化分析

    The influence of methylation on the promoter activity and gene expression and th

    2024-09-24 25
  • 分子实验方法7: RFLP和RAPD技术

    分子实验方法7: RFLP和RAPD技术

    第七章 RFLP和RAPD技术 第一节 概 述  DNA分子水平上的多态性检测技术是进行基因组研究的基础。RFLP(Restriction Fragment L

    2024-09-24 37
  • CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA

    CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA

    DAY 1: 1) Reprecipitate DNA'sAdd the following DNA's to a 1.5 ml centrif

    2024-09-24 22
  • CGH Protocols (四)

    CGH Protocols (四)

    CGH Image acquisitionImages were acquired through a Zeiss Axiophot fluorescence

    2024-09-24 22