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蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrysta
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Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These ce
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蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
Direct Enzymatic Digestion of Protein ComplexesSherry Niessen, Ian McLeod and Jo
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Home-made Taq Polymerase Purification
Home-made Taq Polymerase PurificationI also have the bacteria containing the clo
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ELISA FOR DETECTING SECRETED ICAM-1
PurposeMaterialsNUNC-Immuno Plate IIF.Horseradish Peroxidase-Streptavidin (Zymed
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WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contai
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Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions In
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Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
IntroductionThis is an adaptation of the published procedure for RNAse treatment
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Western Blot Protocols
Western Blot MaterialsSemi-dry Transfer ApparatusBiorad, Cat# 170-3940, or equiv
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Protocol for anti-HA antibody Western Blotting
1) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of 1litre)
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GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarso
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运用肽库筛选磷酸化激酶motif方法 Screening Kinase Phosphorylation Motifs Using Peptide Libraries
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke an
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Synthesis and Probing of Membrane-bound Peptide Arrays
Synthesis and Probing of Membrane-bound Peptide Arrays Ronald Fra
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氰化高铁血红蛋白测定法
[目的与原理]应用氰化高铁血红蛋白(HiCN)法,测定不同鱼类血红蛋白含量。血红蛋白(Hb)被高铁氰化钾[K 3 Fe(CN) 6 ]氧化为高铁血红蛋白(Hi)
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动物血中超氧化物歧化酶的提取
[原理] l969年,McCord和Fridovich第一次从牛血中提纯到超氧化物岐化酶。 自然界中SOD分布极广,其含量随生物体的不同而不同,即使同一种生物的
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