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RNA 操作注意事项与实验技巧
操作注意事项： 由于RNA酶的广泛存在与难以灭活的顽固特性。使得RNA的提取纯化和后续工作变得非常困难。为了保证RNA的研究工作成功，请仔细阅读下列注意事项
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TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
Harvest tissue and rinse in PBS or saline. Immerse tissue in 4% paraformaldehyde
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Northern Blotting (Breeden Lab)
Northern Blotting (Breeden Lab)关键词： northern blotting breeden lab来源： 互联网I. Elect
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Northern Blots
Northern Blotsby Michael Koelle and Tory Herman, adapted from Sambrook et al., &
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Northern杂交试验指导手册－RNA质量检测
二、RNA质量检测 提取的RNA需要对其质量和数量进行检测。实验室常用的方法有紫外吸收值检测和电泳检测两种。 方法一、紫外吸收检测 试剂：TE ( 10mmol
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Northern Blot
I. Electrophoresisclean gel box with NaOH and/or SDS, 2 hours to overnight, rin
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DROSOPHILA RNA PREP
DROSOPHILA RNA PREP (Goodman Lab)Stock Solutions3M NaOAc, pH 5.2 with HAc (MW=82
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Lentiviral strategies for RNAi gene knockdown
IntroductionAmong the 30-40 thousand protein-encoding genes in the human genome,
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Southern Hybridazation
HybridizationMaterials Prehybridization Solution (10 - 50 mL). Mix by rotating
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RNA酶保护试验((RNase Protection Assay,RPA)简介
RNA酶保护试验((RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交，以检测RNA表达的技术。 1。原理：
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推荐：RNAi技术在功能基因组中的应用
在功能基因组研究中，需要对特定基因进行功能丧失或降低突变，以确定其功能。由于RNAi具有高度的序列专一性，可以特异地使特定基因沉默，获得功能丧失或降低突变，因此
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Northern blotting - gel preparation and treatment(Northern杂交-凝胶制备及处理)
RNA is separated under denaturing conditions; the principal systems currently in
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RNA干扰在慢性疼痛治疗中的应用
第二军医大学东方肝胆外科医院麻醉科 吴飞翔 综述 俞卫锋 审校 摘要 慢性疼痛的治疗临床上效果不佳，集中发展更多有效的治疗方法已经成为一个普遍的主题。RNA干
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WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
June, 1997 - optimized by N. Kertesz, L.Leyns & E. De RobertisPlease cite: Belo
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In situ hybridization of yeast cells (RNA and Oligonucleotide probes)
I. General considerations:In situ hybridization of yeast cells is almost identic
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