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siRNAs可以发挥miRNAs功能
Doench JG, Petersen CP, Sharp PA. (2003) siRNAs can function as miRNAs. Genes De
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Agarose-Formaldehyde Electrophoresis
Agarose-Formaldehyde ElectrophoresisRNA electrophoresis under denaturing conditi
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Protein-DNA Telomere FISH
Protein-DNA Telomere FISH FIX AND PROTEIN STRAIN:Fix cells in 4% paraformaldhyde
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Northern Blotting: Efficient RNA Staining and Transfer
(original protocol by R. M. Fourney, S. Miyakoshi, R. S. Day III, and M. C. Pete
2024-11-02 23 -
Pre-hyb/hyb for Radioactive Probes
Pre-hyb/hyb for Radioactive ProbesStuff you need:50X Denhardt's reagent:1% F
2024-11-02 23 -
RNAi-制备siRNAs的方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
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The Neverfail Northern Blot Hybridization
Prehybridization/Hybridization Solution 0.1% SDS 50% Formamide 5x SSC 50 mM NaP
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E.coli Total RNA Labeling Protocol for Spotted Microarray
Note: Start with 20 mg of total RNA for each labeling reaction. All solutions th
2024-11-02 23 -
Hiro Hirai PRINS Protocol
Hiro Hirai 's PRINS ProtocolOverviewThe PRINS (Primed in situ ) technique us
2024-11-02 23 -
[Nature] RNAi研究专题
Cells utilize RNA interference (RNAi) to regulate protein expression in many con
2024-11-02 29 -
Primer Extension analysis of total yeast RNA
Primer Extension analysis of total yeast RNA1. Mix the following in a 1.5 ml epp
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Northern Blotting (Howell Lab)
RNA isolationHarvest cells when they are about 80-85% confluent. Stationary phas
2024-11-02 29 -
RNA干扰沉默癌基因
RNA interference silences cancer geneDavid Douglas Reuters Health Posting Date:
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Collection of Tissue for RNA Analysis
Handling RNA: Ribonucleases (RNases) are very stable and active enzymes that deg
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Decontamination and inhibition of ribonucleases
Decontamination and inhibition of ribonucleasesIt is common knowledge in most la
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