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Northern杂交试验指导手册－RNA提取
方法一、改良异硫氰酸胍提取法 试剂及其配制 异硫氰酸胍变性液： 贮存液－在含484ml 水（经DEPC处理）, 17.6ml 0.75mol/L柠檬酸钠（pH7
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What is your Protocol for RNAi on Cell Cultures
6-Well Plates Bathing Prepare dsRNA suspended in water. We use ~500 bp dsRNA. Ad
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Northern Hybridization of RNA Fractionated by Agarose-formaldehyde Gel
Northern Hybridization of RNA Fractionated by Agarose-formaldehyde GelAuthor: Zu
2024-11-02 28 -
Increasing Your RNA Recovery During Tissue or Cell Extraction
One of the most problematic steps in RNA isolation is the first step - thorough
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NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGEN
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTPFor the use with
2024-11-02 25 -
地高辛标记cRNA探针实验方法
1．组织前处理在组织制片中以冷冻切片（厚10～30μm）最佳。切片贴在预先清洁，高温处理并涂以粘附剂载玻片上，先在37℃预干燥4h，然后置于37℃烤箱中过夜。经
2024-11-02 27 -
RNA干扰及其应用进展
孙德惠 才学鹏 常惠芸 独军政 （1.中国农业科学院兰州兽医研究所家畜疫病病原 生物 学国家重点实验室农业部畜禽病毒学重点开放实验室，甘肃兰州 730046；2
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新RNAi技术成功抑制人的基因表达
这篇报道有关采用RNAi技术在哺乳动物中大规模应用的相关报道。主要说明该技术将来的应用前景。原文请见下面的相关报道。New RNA libraries can
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Northerns
NorthernsStuff you need:10X running buffer:50 mM NaOAc0.2M MOPS pH 7.010 mM EDTA
2024-11-02 26 -
RNA干扰研究前沿和应用领域
小RNA（MicroRNA，miRNA）的作用与应用研究继2001，2002连续两年被美国《Science》杂志评选为年度10大突破技术以来，2003年继续热度
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RNA干扰:肿瘤治疗的新领域
RNA干扰（RNA interference RNAi）现象最先发现于秀丽杆线虫，这是一种由双链RNA（double-stranded RNA, dsRNA）诱
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Northern Blots(Ambros Lab)
by Michael Koelle and Tory Herman, adapted from Sambrook et al., "Molecular
2024-11-02 20 -
Northern杂交试验指导手册－杂交结果检测
方法一、化学发光检测 试剂及其配制： 洗膜缓冲液：100mmol Maleic acid 150mmol NaCl 0.3%(v/v) Tween20 pH 7
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In situ hybridization of mammalian cells (RNA and Oligonucleotide p
I. General ConsiderationsIn situ hybridization is a powerful technique which can
2024-11-02 26 -
mRNA Purification
mRNA Purification I. Prepare oligo-dT celluloseuse 40 mg oligo-dT cellulose /
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